Anti-influenza virus effects of crude phenylethanoid glycosides isolated from ligustrum purpurascens via inducing endogenous interferon-γ
Anti-influenza virus effects of crude phenylethanoid glycosides isolated from ligustrum purpurascens via inducing endogenous interferon-γ
复制标题
从女贞中分离的粗苯乙醇苷通过诱导内源性干扰素-γ的抗流感病毒作用。
DOI：
10.1016/j.jep.2015.07.019
复制
发表时间：
2016-02-17
影响因子：
5.4
通讯作者：
He, Zhen-dan
中科院分区：
文献类型：
--
作者：
Hu, Xiao-peng;Shao, Min-ming;He, Zhen-dan
民族药理学相关性：紫女贞Y.C.杨（木犀科）传统上被记录为“苦丁茶”，是一种在中国南方有大约两千年历史的功能茶，据报道具有缓解咽喉疼痛和清热祛邪的作用。然而，没有科学研究证明其抗病毒活性。本研究的目的是：研究从L.材料与方法：体外血凝试验检测流感病毒滴度;体内给C57 BL/6 J小鼠灌胃给予100 mg/kg、300 mg/kg、900 mg/kg三种剂量的猪流感病毒颗粒（LPG）或利巴韦林（100 mg/kg），每日1次，连续5 d。同时，在第二天，将小鼠鼻内（i. n.）A/FM/1/47 H1N1病毒。连续观察15 d，观察小鼠存活率及其他临床指标。处死感染的小鼠以测量肺病变并用苏木精染色。流式细胞术分析脾淋巴细胞和干扰素-γ（IFN-gamma）水平。结果：在体外，0.5mg/ml的LPG可抑制甲型流感病毒H1N1型（H1N1）感染MDCK细胞，并能显著抑制H1N1病毒对MDCK细胞的感染。在体内，300和900 mg/kg的LPG显著降低小鼠肺指数（p < 0.05），减轻流感诱导的致死率和临床症状，因此提高小鼠存活率（p < 0.05）。
Ethnopharmacological relevance: Ligustrum purpurascens Y.C. Yang (Oleaceae) is traditionally recorded as "Ku Ding Cha", a kind of functional tea in southern China for about two thousand years, which has been reported with sore throat alleviating and pathogenic heat expelling effects. However, there are no scientific studies demonstrating its antiviral activity. The aim of the study: This study is aimed at investigating the anti-influenza virus effects of phenylethanoid glycosides isolated from L. purpurascens (LPG) as well as its corresponding mechanisms.Materials and methods: In vitro, hemagglutination assay was employed to detect the influenza virus titer; In vivo, C57BL/6J mice were given oral administration of LPG (100 mg/kg, 300 mg/kg, 900 mg/kg) or ribavirin (100 mg/kg) once daily for 5 successive days. Meanwhile, on the second day, mice were infected intranasally (i.n.) with A/FM/1/47 H1N1 virus. Mice survival rate and other clinical index were monitored for 15 days. Infected mice were sacrificed to measure the lung lesion and stained with hematoxylineosin. Flow cytometry analyses spleen lymphocytes and interferon-gamma (IFN-gamma) level. The IFN-gamma knockout mice (IFN-gamma(-/-) mice, C57BL/6J) which had been verified lacking IFN-gamma through Western Blot, were applied in the death-protection test to identify the role of IFN-gamma played in LPG antiviral effect.Results: In vitro, LPG at 0.5 mg/ml inhibited Influenza A Virus H1N1 type (H1N1) infection of MDCK cells. In vivo, LPG at 300 and 900 mg/kg significantly decreased the mouse lung index (p < 0.05), alleviated influenza-induced lethality and clinical symptoms, and therefore enhanced mouse survival (p