Polyamines produced by an extreme thermophile are essential for cell growth at high temperature
Polyamines produced by an extreme thermophile are essential for cell growth at high temperature
复制标题
DOI：
10.1093/jb/mvac048
复制
发表时间：
2022-05-25
影响因子：
2.7
通讯作者：
Terui, Yusuke
中科院分区：
文献类型：
--
作者：
Sakamoto, Akihiko;Tamakoshi, Masatada;Terui, Yusuke
嗜热菌是一种极端嗜热菌，在70摄氏度左右的最适温度下生长，产生16种不同的多胺，包括长链和支链多胺。我们发现，嗜热细胞中多胺的组成随培养温度的变化而变化。在高温(如80℃)下生长的细胞中，长链和支链多胺(异常多胺)增加，但在相对较低的温度(如60℃)下生长的细胞中，它们是次要成分。该突变株的细胞生长在70℃时显著下降，不能产生多胺，在75℃下生长不良。此外，还确定了多胺是否直接参与保护DNA免受热诱导的DNA双链断裂(DSB)。多胺保护DNA免受双链断裂。因此，多胺通过维持DNA对DSB的功能性构象和去嘌呤作用，在极高温下对细胞的生长起着至关重要的作用。
An extreme thermophile, Thermus thermophilus grows at an optimum temperature of around 70 degrees C and produces 16 different polyamines including long-chain and branched-chain polyamines. We found that the composition of polyamines in the thermophile cells changes with culture temperature. Long-chain and branched-chain polyamines (unusual polyamines) were increased in the cells grown at high temperature such as 80 degrees C, but they were minor components in the cells grown at relatively lower temperature such as 60 degrees C. The effects of polyamines on cell growth were studied using T. thermophilus HB8 Delta speA deficient in arginine decarboxylase. Cell growth of this mutant strain was significantly decreased at 70 degrees C. This mutant strain cannot produce polyamines and grows poorly at 75 degrees C. It was also determined whether polyamines are directly involved in protecting DNA from DNA double-strand breaks (DSBs) induced by heat. Polyamines protected DNA against double-strand breaks. Therefore, polyamines play essential roles in cell growth at extremely high temperature through maintaining a functional conformation of DNA against DSBs and depurination.