Calcium Spikes in Epithelium: study on Drosophila early embryos.
Calcium Spikes in Epithelium: study on Drosophila early embryos.
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DOI：
10.1038/srep11379
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发表时间：
2015-07-22
影响因子：
4.6
通讯作者：
Lenne PF
中科院分区：
文献类型：
--
作者：
Markova O;Sénatore S;Chardès C;Lenne PF
钙离子几乎作用于细胞生命的各个方面。这种普遍存在的作用所需的多功能性和特异性是由钙浓度变化的时空动力学来保证的。虽然钙信号动力学已被广泛研究，在细胞培养和成人组织中，很少有人知道在早期组织形态发生的钙活性。我们监测了果蝇原肠胚细胞内钙浓度，发现单细胞钙峰是短暂的，罕见的，并表现出很强的变异性胚胎。我们定量描述了这些尖峰的时空动力学，并通过引入物理和化学扰动分析了它们的潜在来源和性质。我们的数据突出了组织形态发生过程中钙活性的组织间和组织内变异性。
Calcium ion acts in nearly every aspect of cellular life. The versatility and specificity required for such a ubiquitous role is ensured by the spatio-temporal dynamics of calcium concentration variations. While calcium signal dynamics has been extensively studied in cell cultures and adult tissues, little is known about calcium activity during early tissue morphogenesis. We monitored intracellular calcium concentration in Drosophila gastrula and revealed single cell calcium spikes that were short-lived, rare and showed strong variability among embryos. We quantitatively described the spatio-temporal dynamics of these spikes and analyzed their potential origins and nature by introducing physical and chemical perturbations. Our data highlight the inter- and intra-tissue variability of calcium activity during tissue morphogenesis.