The homologous HD-Zip I transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins
The homologous HD-Zip I transcription factors HaHB1 and AtHB13 confer cold tolerance via the induction of pathogenesis-related and glucanase proteins
复制标题
DOI：
10.1111/j.1365-313x.2011.04778.x
复制
发表时间：
2012-01-01
期刊：
影响因子：
7.2
通讯作者：
Chan, Raquel L.
中科院分区：
文献类型：
--
作者：
Cabello, Julieta V.;Arce, Agustin L.;Chan, Raquel L.
植物通过不同的信号转导途径来应对低温。向日葵的Hd-Zip I同源转录因子HaHB1和拟南芥的AtHB13在这种冷反应中起着关键作用。分析了这两个基因的表达模式，表明低温上调了这两个基因的表达。当这些基因在拟南芥中组成性表达时，转基因植株表现出相似的表型，包括细胞膜在冷冻处理下的稳定性和耐冷性。对HaHB1转基因植株的探索性转录分析表明，诱导出了几个编码葡聚糖酶和几丁质酶的转录本。此外，在冷冻条件下，一些蛋白质在HaHB1植物质外体中积累，这些提取物在体外具有抗冻活性。三个编码两个葡聚糖酶和一个几丁质酶的基因在拟南芥中过表达，这些植物能够耐受冰冻温度。所有获得的转基因植株在短暂的冷冻处理后都表现出细胞膜的稳定。最后，利用HaHB1和AtHB13瞬时转化向日葵和大豆，上调了HaHB1/AtHB13同源基因的表达，从而表明冷反应途径的保守性。我们认为，HaHB1和AtHB13通过诱导能够稳定细胞膜和抑制冰生长的蛋白质参与植物的耐冷性。
Plants deal with cold temperatures via different signal transduction pathways. The HD-Zip I homologous transcription factors HaHB1 from sunflower and AtHB13 from Arabidopsis were identified as playing a key role in such cold response. The expression patterns of both genes were analyzed indicating an up-regulation by low temperatures. When these genes were constitutively expressed in Arabidopsis, the transgenic plants showed similar phenotypes including cell membrane stabilization under freezing treatments and cold tolerance. An exploratory transcriptomic analysis of HaHB1 transgenic plants indicated that several transcripts encoding glucanases and chitinases were induced. Moreover, under freezing conditions some proteins accumulated in HaHB1 plants apoplasts and these extracts exerted antifreeze activity in vitro. Three genes encoding two glucanases and a chitinase were overexpressed in Arabidopsis and these plants were able to tolerate freezing temperatures. All the obtained transgenic plants exhibited cell membrane stabilization after a short freezing treatment. Finally, HaHB1 and AtHB13 were used to transiently transform sunflower and soybean leading to the up-regulation of HaHB1/AtHB13-target homologues thus indicating the conservation of cold response pathways. We propose that HaHB1 and AtHB13 are involved in plant cold tolerance via the induction of proteins able to stabilize cell membranes and inhibit ice growth.