Soybean β-conglycinin diet suppresses serum triglyceride levels in normal and genetically obese mice by induction of β-oxidation, downregulation of fatty acid synthase, and inhibition of triglyceride absorption
Soybean β-conglycinin diet suppresses serum triglyceride levels in normal and genetically obese mice by induction of β-oxidation, downregulation of fatty acid synthase, and inhibition of triglyceride absorption
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DOI：
10.1271/bbb.68.352
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发表时间：
2004-02-01
影响因子：
1.6
通讯作者：
Maebuchi, M
中科院分区：
文献类型：
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作者：
Moriyama, T;Kishimoto, K;Maebuchi, M
本研究旨在探讨大豆β-伴大豆球蛋白（7S-球蛋白）和大豆球蛋白（11 S-球蛋白）对正常和遗传性肥胖小鼠血脂水平和肝脏代谢的影响。雄性正常（ICR）和肥胖（KK-A（y））小鼠自由进食高脂肪饮食两周，随后限制进食含有20%酪蛋白、大豆β-伴大豆球蛋白或大豆球蛋白的饮食（2 g饮食/小鼠/天）2周，然后立即处死。血清甘油三酯（TG），葡萄糖和胰岛素水平的β-伴大豆球蛋白喂养的小鼠低于酪蛋白和大豆球蛋白喂养的小鼠的两个菌株。为了分析与这些效应相关的事件，测定了脂代谢相关蛋白的酶活性和相对mRNA水平。与脂肪酸β-氧化相关的两种酶的活性在β-伴大豆球蛋白喂养的小鼠的肝脏中高于酪蛋白喂养的小鼠，而脂肪酸合成酶的活性在这两种小鼠的肝脏中低于酪蛋白喂养的小鼠。β-伴大豆球蛋白喂养小鼠肝脏中酰基辅酶A氧化酶（脂肪酸β-氧化相关酶）的信使RNA水平显著高于酪蛋白喂养小鼠。相反，SREBP-1和2的mRNA水平在大豆蛋白喂养的小鼠的肝脏中倾向于降低比两种酪蛋白喂养的小鼠。β-伴大豆球蛋白喂养小鼠的TG粪便排泄量高于酪蛋白喂养小鼠。我们的研究结果表明，大豆β-伴大豆球蛋白饮食通过加速β-氧化、抑制脂肪酸合成酶和/或增加TG粪便排泄来降低血清TG水平，并且还降低血清葡萄糖和胰岛素水平。其中一些事件可能是在转录水平上引起的，从脂质代谢相关蛋白的相对信使RNA水平改变的结果判断。这些结果表明，大豆β-伴大豆球蛋白可能是一种潜在的有用的膳食蛋白质来源，用于预防高脂血症，高胰岛素血症和高血糖症，这些被认为是动脉粥样硬化的危险因素。
The purpose of this study was to discover the effects of soybean beta-conglycinin (7S-globulin) and glycinin (11S-globulin) on serum lipid levels and metabolism in the livers of normal and genetically obese mice. Male normal (ICR) and obese (KK-A(y)) mice were fed ad libitum high fat diets for two weeks, followed by a 2-week restriction of diet (2 g diet/mouse/day) containing 20% casein, soybean beta-conglycinin, or soybean glycinin, and then sacrificed immediately. Serum triglyceride (TG), glucose, and insulin levels of beta-conglycinin-fed mice were lower than in casein- and glycinin-fed mice of both strains. In order to analyze the related events to these effects, enzyme activities and relative mRNA levels of lipid metabolism-related proteins were measured. The activities of two enzymes related to fatty acid beta-oxidation were higher while that of fatty acid synthase was lower in livers of beta-conglycinin-fed mice than of casein-fed both mice. Messenger RNA levels of acyl-CoA oxidase (fatty acid beta-oxidation related enzyme) were significantly higher in livers of beta-conglycinin-fed mice than of both casein-fed mice. On the contrary, mRNA levels of SREBP-1 and 2 tended to be lowered in livers of soy protein-fed mice than of both casein-fed mice. Fecal excretion of TG was higher in beta-conglycinin-fed mice than in casein-fed mice. Our results demonstrated that the soy beta-conglycinin diet reduced serum TG levels by acceleration of beta-oxidation, suppression of fatty acid synthase and/or increased TG fecal excretion, and also diminished serum glucose and insulin levels. Some of these events might be caused at the transcriptional levels, judged from the result that relative messenger RNA levels of lipid metabolism-related proteins were altered. These results suggest that soy beta-conglycinin could be a potentially useful dietary protein source for the prevention of hypertriglyceridemia, hyperinsulinemia, and hyperglycemia, which are recognized as risk factors for atherosclerosis.