Hybridization-based unquenching of DNA hairpins on Au surfaces: Prototypical "molecular beacon" biosensors
Hybridization-based unquenching of DNA hairpins on Au surfaces: Prototypical "molecular beacon" biosensors
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DOI：
10.1021/ja0290781
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发表时间：
2003-04-09
影响因子：
15
通讯作者：
Krauss, TD
中科院分区：
文献类型：
--
作者：
Du, H;Disney, MD;Krauss, TD
人们对开发不需要标记分析物的快速遗传分析技术非常感兴趣。在这里，我们证明了荧光标记的DNA发夹连接到金膜可以作为固定的“分子信标”。将金黄色葡萄球菌FemA和mecR耐甲氧西林基因的两个DNA发夹结合到金基底上。在暴露于补体后，测量到荧光强度增加26倍，对应于96 ± 5%的淬灭效率。对非特异性DNA的研究表明，固定在金表面上的DNA发夹保留了它们选择性结合互补DNA序列的能力。
There is a keen interest in developing techniques for rapid genetic analysis that do not require labeling of an analyte. Here we demonstrate that fluorophore-tagged DNA hairpins attached to gold films can function as immobilized “molecular beacons”. Two DNA hairpins incorporating portions of theStaphlococcus aureusFemAandmecRmethicillin-resistance genes were attached to a gold substrate. Upon exposure to the complement, a ∼26-fold increase in fluorescence intensity was measured corresponding to a 96 ± 5% quenching efficiency. Studies with nonspecific DNA indicate that DNA hairpins immobilized on a gold surface retain their ability to bind complementary DNA sequences selectively.