Structural genomics and its importance for gene function analysis
Structural genomics and its importance for gene function analysis
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DOI：
10.1038/73723
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发表时间：
2000-03-01
影响因子：
46.9
通讯作者：
Kolinski, A
中科院分区：
文献类型：
--
作者：
Skolnick, J;Fetrow, JS;Kolinski, A
结构基因组学项目旨在解决所有可能的蛋白质折叠的实验结构。这些项目需要从传统的结构生物学概念上的转变，在结构生物学中，获得已知蛋白质的结构信息，在结构生物学中，首先确定蛋白质的结构，然后才分配功能。虽然将蛋白质结构转换为功能的目标可以通过传统的基于序列基序的方法来实现，但最近的研究表明，蛋白质的生化功能的分配也可以通过扫描其结构以匹配已知活性位点的几何形状和化学特性来实现。重要的是，这种方法可以使用当代结构预测方法提供的低分辨率结构。当应用于基因组时，结构信息（无论是实验的还是预测的）可能在高通量功能分配中发挥重要作用。
Structural genomics projects aim to solve the experimental structures of all possible protein folds. Such projects entail a conceptual shift from traditional structural biology in which structural information is obtained on known proteins to one in which the structure of a protein is determined first and the function assigned only later. Whereas the goal of converting protein structure into function can be accomplished by traditional sequence motif-based approaches, recent studies have shown that assignment of a protein's biochemical function can also be achieved by scanning its structure for a match to the geometry and chemical identity of a known active site. Importantly, this approach can use tow-resolution structures provided by contemporary structure prediction methods. When applied to genomes, structural information (either experimental or predicted) is likely to play an important role in high-throughput function assignment.