Analysis of flavonoids in pubescence of soybean near-isogenic lines for pubescence color loci
Analysis of flavonoids in pubescence of soybean near-isogenic lines for pubescence color loci
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DOI：
10.1093/jhered/esl027
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发表时间：
2006-09-01
影响因子：
3.1
通讯作者：
Takahashi, Ryoji
中科院分区：
文献类型：
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作者：
Iwashina, Tsukasa;Benitez, Eduardo R.;Takahashi, Ryoji
T和Td基因座通过上位性效应控制大豆的毛色（TT TdTd，黄褐色; TT tdtd，浅黄褐色或近灰色; it TdTd或tt tdtd，灰色）。本研究的目的是调查这些位点的近等基因罚款（NILs）的短柔毛类黄酮的性质。用甲醇从具有黄褐色短柔毛的品种Clark（TT TdTd）及其近等基因系的短柔毛、具有灰色短柔毛的Clark-t（tt TdTd）和具有近灰色短柔毛的Clark-td（TT tdtd）以及具有黄褐色短柔毛的To 7 B（TT TdTd）和具有灰色短柔毛的To 7 G（tt TdTd）的一对近等基因系的短柔毛中提取黄酮。主要黄酮类化合物为黄酮苷元。木犀草素和芹菜素分别在黄褐色和灰色短柔毛的近等基因系中占优势。两种黄酮类化合物均含有少量的7-O-葡萄糖苷。T位点的等位基因与黄酮B环的3 '-羟基化有关。Clark-td中的主要黄酮类化合物是类似于Clark的木犀草素，但其含量减少了一半。2003年仅在Clark-td中发现了可能对应于甘草酸的高效液相色谱峰。然而，2005年没有观察到峰值。上述结果表明，Td可能编码一个控制黄酮生物合成的结构或调控基因。甲醇提取后，色素在绒毛中仍然可见，表明大部分色素是聚合的。表面漂洗实验表明，黄酮苷元存在于细胞表面之外。
T and Td loci control pubescence color of soybean with epistatic effects (TT TdTd, tawny; TT tdtd, light tawny or near-gray; it TdTd or tt tdtd, gray). The objective of this study was to investigate the nature of flavonoids in the pubescence of near-isogenic fines (NILs) for these loci. Flavonoids were extracted with methanol from pubescence of cultivar Clark with tawny pubescence (TT TdTd) and its NILs; from Clark-t with gray pubescence (tt TdTd) and Clark-td with near-gray pubescence (TT tdtd); and from a pair of NILs, To7B with tawny (TT TdTd) and To7G with gray pubescence (tt TdTd). Primary flavonoids were flavone aglycones. Luteolin and apigenin were predominant in NILs with tawny and gray pubescence, respectively. Small amount of 7-O-glucosides of the 2 flavones were also detected. Alleles at T locus were associated with 3'-hydroxylation in the B-ring of the flavones. The primary flavonoids in Clark-td were luteolin similar to Clark, but its amount was halved. High performance liquid chromatography peaks probably corresponding to isoflavonoids were found only in Clark-td in 2003. However, the peaks were not observed in 2005. The above results suggest that Td may encode a structural or a regulatory gene controlling flavone biosynthesis. Pigments remained visible in pubescence after methanol extraction, suggesting that a major part of the pigments was polymerized. Surface rinsing experiments revealed that flavone aglycones exist outside the surface of cells.