Early colonic damage and invasion of Campylobacter jejuni in experimentally challenged infant Macaca mulatta.
Early colonic damage and invasion of Campylobacter jejuni in experimentally challenged infant Macaca mulatta.
复制标题
实验挑战婴儿猕猴中的早期结肠损伤和空肠弯曲杆菌的入侵。
DOI：
10.1093/infdis/168.1.210
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
DeTolla,LJ
中科院分区：
文献类型：
--
作者：
Russell,RG;O'Donnoghue,M;BlakeJr,DC;Zulty,J;DeTolla,LJ
用空肠弯曲菌对3、5月龄恒河猴幼仔进行了攻毒试验。用2.7 × 1010 cfu菌株78-37攻毒1只幼猴（92-1）。第二个婴儿是通过自然传播故意感染的。婴儿92-1攻毒后32 h出现腹泻。电镜观察表明，细胞侵袭是C.空肠。胞内C.空泡位于膜泡中，游离于细胞质中。损伤的上皮细胞表现出过早的凋亡，并脱落到结肠腔中。空肠上皮细胞也位于粘膜和粘膜下层的细胞外。有些细胞有扩张的内质网，表明可能改变离子和水的运输。
Experimental challenge studies withCampylobacter jejuniwere conducted in 3,5-month-old infantMacaca mulatta. One infant monkey (92-1) was challenged with 2.7 × 1010cfu of strain 78-37. A second infant was infected intentionally by natural transmission. The infants developed diarrhea 32 h after challenge of infant 92-1. Electron microscopic observations indicate that cell invasion is the primary mechanism of colon damage and diarrheal disease caused byC. jejuni. IntracellularC. jejuniwere located in membrane-bound vacuoles and were free in the cytoplasm. Damaged epithelial cells exhibited premature apoptosis and were exfoliated into the lumen of the colon.C. jejuniwere also located extracellularly in the mucosa and submucosa. Some cells had dilated endoplasmic reticulum, indicating possible alteration in ion and water transport.