Isolation of Hemagglutinative Non-El Tor Cholera Vibrios
Isolation of Hemagglutinative Non-El Tor Cholera Vibrios
复制标题
血凝非 El Tor 霍乱弧菌的分离
作者：
S. Rizvi;M. Huq;A. Benenson
1963年期间，埃尔托霍乱在泰国和缅甸蔓延，最终于11月出现在东巴基斯坦的吉大港地区（Yen等，东巴基斯坦失踪。J. 8：22，1964）。在本实验室研究的80 km范围内由El Tor弧菌引起的疾病中，通过Finkelstein和AIukerjee提出的鸡红细胞凝集试验（Proc. Soc. Exp.生物学M1 ed。112：355，1963）以区分El Tor与经典霍乱弧菌。1964年1月10日，从6名疑似霍乱患者中收到了碱性亚碲酸盐胆汁蛋白胨肉汤中的直肠拭子。这些拭子由一名政府卫生访视员于1月6日从该实验室疫苗研究区附近的一个村庄获得。从所有拭子中分离出稻叶霍乱弧菌（V. comma）;在Finkelstein和MIukerjee玻片凝集试验中，其中两种培养物的琼脂表面生长凝集鸡细胞。在随后的6周内，从384个其他个体中分离出O组I可凝集弧菌。从六个人，血凝弧菌，获得其中三个，一个nonhemagglutinative弧菌被分离在同一时间作为血凝性的。从先前研究的750个个体中分离的霍乱弧菌培养物中未发现血凝性菌株。直到1964年9月13日，从另外160个个体（17个Ogawa和143个Inaba）中分离出霍乱弧菌培养物，但没有一个是血凝性的，尽管对这种弧菌进行了特别的搜索。66个典型的小川血清型埃尔托尔弧菌菌株在这后一时期收到的各种其他实验室都是血凝性。因为1963年11月在东巴基斯坦分离出了典型的埃尔托弧菌。这八种血凝性菌株被认为预示着埃尔托霍乱在我们研究区域内的出现。因此，这些菌株与美国印刷的第89卷第3号非血凝性菌株一起
During 1963, El Tor cholera spread through Thailand and Burma, and finally appeared in the Chittagong area of East Pakistan in November (Yen et al., East Pakistan MIed. J. 8:22, 1964). With disease caused by the El Tor vibrio within 80 km of areas under study by this laboratory, all vibrio isolates which agglutinated in cholera antisera were screened by the chicken erythrocyte agglutination test proposed by Finkelstein and AIukerjee (Proc. Soc. Exp. Biol. M1ed. 112:355, 1963) to differentiate El Tor from classic cholera vibrios. On 10 January 1964, rectal swabs were received in alkaline tellurite bile peptone broth from six individuals with presumed cholera. These swabs had been obtained by a government health visitor on 6 January from a village adjacent to the vaccine study area of this laboratory. From all swabs, Vibrio cholerae Inaba (V. comma) was isolated; agar-surface growth of two of these cultures agglutinated chicken cells in the Finkelstein and MIukerjee slide agglutination test. During the ensuing 6 weeks, O group I agglutinable vibrios were isolated from 384 other individuals. From six individuals, hemagglutinative vibrios were obtained; in three of these, a nonhemagglutinative vibrio was isolated at the same time as the hemagglutinative one. No hemagglutinative strains had been found among the V. cholerae cultures isolated from 750 individuals previously studied. UTntil 13 September 1964, V. cholerae cultures were isolated from an additional 160 individuals (17 Ogawa and 143 Inaba), but none was hemagglutinative, despite a special search for such vibrios. Sixty-six typical Ogawa serotype El Tor vibrio strains received during this latter period from various other laboratories were all hemagglutinative. Because typical El Tor vibrios had been isolated in East Pakistan in November 1963. the eight hemagglutinative strains were believed to herald the appearance of El Tor cholera within our study area. Accordingly, these strains, together with the nonhemagglutinative strains Vol. 89, No. 3 Printed in U.S.A.