Gene Expression Enabling Synthetic Diversification of Natural Products: Chemogenetic Generation of Pacidamycin Analogs
Gene Expression Enabling Synthetic Diversification of Natural Products: Chemogenetic Generation of Pacidamycin Analogs
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DOI：
10.1021/ja1060406
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发表时间：
2010-09-08
影响因子：
15
通讯作者：
Goss, Rebecca J. M.
中科院分区：
文献类型：
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作者：
Roy, Abhijeet Deb;Grueschow, Sabine;Goss, Rebecca J. M.
引入prnA，卤化酶基因从吡咯硝基生物合成，到链霉菌蓝淡红导致有效的原位氯化的尿苷肽抗生素帕卡霉素。安装的氯提供了一个可选择的官能化处理，使天然产物的合成改性，使用温和的交叉偶联条件下，在粗含水提取物的培养液。
Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.