Purification and Properties of Oxidized Poly(vinyl alcohol)- Degrading Enzyme
Purification and Properties of Oxidized Poly(vinyl alcohol)- Degrading Enzyme
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DOI：
10.1271/bbb1961.45.63
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发表时间：
1981-07
期刊：
影响因子：
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通讯作者：
K. Sakai;M. Morita;N. Hamada;Yasuto Watanabe
中科院分区：
文献类型：
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作者：
K. Sakai;M. Morita;N. Hamada;Yasuto Watanabe
将催化仲醇氧化酶氧化的聚（乙烯醇）降解的酶从至少三种不同土壤细菌的混合培养液中纯化至电泳均质状态，其中聚（乙烯醇）的羟基部分转化为酮基。该酶对氧化的聚（乙烯醇）有活性，但对完整的聚（乙烯醇）和各种检查的低分子量酮化合物没有活性。因此，该酶暂称为氧化聚(乙烯醇)降解酶。该酶是分子量为38,000的单一多肽。 N端和C端氨基酸分别是丙氨酸和苏氨酸。等电点为pH 10.0。最适活性pH为6.5，最适温度为45℃。酶活性受到 Hg2+ 的抑制，并被还原型谷胱甘肽恢复，但对氯汞苯甲酸盐没有影响。氧化聚（乙烯醇）上的酶反应不需要任何氧化...
An enzyme catalyzing the degradation of secondary alcohol oxidase-oxidized poly(vinyl alcohol), in which hydroxyl groups of poly(vinyl alcohol) are partially converted to keto groups, was purified to an electrophoretically homogeneous state from a mixed culture broth of at least three different soil bacteria. The enzyme was active to the oxidized poly(vinyl alcohol), but not to intact poly(vinyl alcohol) and to a variety of examined low molecular weight keto compounds. The enzyme was, therefore, tentatively called oxidized poly(vinyl alcohol)-degrading enzyme. The enzyme was a single polypeptide having a molecular weight of 38,000. The N- and C-terminal amino acids were alanine and threonine, respectively. The isoelectric point was pH 10.0. The optimum pH for activity was 6.5 and the optimum temperature 45°C. The enzyme activity was inhibited by Hg2+ and recovered by reduced glutathione, although p-chloromercuribenzoate had no effect. The enzyme reaction on oxidized poly(vinyl alcohol) required neither ox...