Glycosylation at an evolutionary nexus: the brittle star Ophiactis savignyi expresses both vertebrate and invertebrate N-glycomic features
Glycosylation at an evolutionary nexus: the brittle star Ophiactis savignyi expresses both vertebrate and invertebrate N-glycomic features
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DOI：
10.1074/jbc.ra119.011703
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发表时间：
2020-03-06
影响因子：
4.8
通讯作者：
Paschinger, Katharina
中科院分区：
文献类型：
--
作者：
Eckmair, Barbara;Jin, Chunsheng;Paschinger, Katharina
棘皮动物是最原始的后口动物之一，由于其与脊索动物的亲缘关系密切，已被用作模式生物来了解脊索动物的生物学。然而，几乎没有关于棘皮动物的N-糖能力的数据，这是否则已知产生一组不同的物种特异性的糖缀合物，包括岩藻糖，硫酸盐和唾液酸残基的严重修改。为了增加这门内的碳水化合物结构的多样性的知识，在这里，我们进行了深入的分析N-聚糖从脆性星星（蛇腹蛇savignyi）作为一个例子成员的纲蛇尾。为此，我们进行了多步N-聚糖分析，通过HPLC和各种外切糖苷酶和化学处理结合MALDI-TOF MS和MS/MS。使用这种方法，我们发现了丰富的混合和复杂的寡糖结构，让人想起那些在高等脊椎动物以及一些经典的无脊椎动物聚糖结构。这些N-聚糖的70%是阴离子的，携带唾液酸、硫酸盐或磷酸盐残基。在糖基发生方面，我们的数据将脆性星星定位于无脊椎动物和脊椎动物之间，并证实了低等生物中N-糖基化的高度多样性。
Echinoderms are among the most primitive deuterostomes and have been used as model organisms to understand chordate biology because of their close evolutionary relationship to this phylogenetic group. However, there are almost no data available regarding the N-glycomic capacity of echinoderms, which are otherwise known to produce a diverse set of species-specific glycoconjugates, including ones heavily modified by fucose, sulfate, and sialic acid residues. To increase the knowledge of diversity of carbohydrate structures within this phylum, here we conducted an in-depth analysis of N-glycans from a brittle star (Ophiactis savignyi) as an example member of the class Ophiuroidea. To this end, we performed a multi-step N-glycan analysis by HPLC and various exoglyosidase and chemical treatments in combination with MALDI-TOF MS and MS/MS. Using this approach, we found a wealth of hybrid and complex oligosaccharide structures reminiscent of those in higher vertebrates as well as some classical invertebrate glycan structures. 70% of these N-glycans were anionic, carrying either sialic acid, sulfate, or phosphate residues. In terms of glycophylogeny, our data position the brittle star between invertebrates and vertebrates and confirm the high diversity of N-glycosylation in lower organisms.