Enhancing proliferation and migration of fibroblast cells by electric stimulation based on triboelectric nanogenerator
Enhancing proliferation and migration of fibroblast cells by electric stimulation based on triboelectric nanogenerator
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基于摩擦纳米发电机的电刺激增强成纤维细胞的增殖和迁移
DOI：
10.1016/j.nanoen.2018.12.077
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发表时间：
2019-03-01
期刊：
影响因子：
17.6
通讯作者：
Pan, Yue
中科院分区：
文献类型：
--
作者：
Hu, Wentao;Wei, Xuelian;Pan, Yue
利用电信号刺激细胞是生物医学发展的重要途径。摩擦电纳米发电机（TENG）能够将机械能转化为电能，提供了一种以所需方式刺激细胞的替代策略。本研究设计了一种TENG驱动的电刺激系统，用于成纤维细胞行为的生物安全性评价和探索。为了获得可调范围的交流输出，制作了一种旋转圆盘形TENG（RD-TENG）。在10-50 μ A范围内产生的峰值电流适合于促进L929细胞的细胞增殖行为。在50 μ A的最佳值下，间歇刺激2天后，促进率达53.8 ± 2.66%。此外，迁移率比对照组中的细胞增加约67%。此外，在TENG刺激下，L929细胞增殖相关基因--增殖细胞核抗原（Pcna）和迁移相关基因--成纤维细胞生长因子2（Fgf 2）和δ样非典型缺口配体1（Dlk 1）表达上调，提示TENG刺激在基因表达水平上调节细胞增殖和迁移。本研究证明了TENG在生物医学刺激中的有效性及其安全操作条件，这为TENG在组织形成、再上皮化和组织重塑方面的实际应用铺平了道路。
Cell stimulation by electric signal is an important approach for the advancement of biomedicine. Triboelectric nanogenerators (TENG), capable of converting mechanical energies to electricity, provides an alternative strategy to stimulate cells in a desired fashion. In this work, a TENG driven electric stimulation system has been designed for biosafety evaluation and exploration of the fibroblast cell behaviors. A rotatory disc-shaped TENG (RD-TENG) was fabricated to obtain an adjustable range of alternating current outputs. The peak current generated in a range of 10-50 mu A is suitable for promoting cellular proliferation behavior of L929 cells. At the optimum value of 50 mu A, the promotion rate reached 53.8 +/- 2.66% after two-day of intermittent stimulation. Also, the migration rate was increased by about 67% than that of the cells in the control group. In addition, under stimulation, the proliferation-related gene of L929 cells - proliferating cell nuclear antigen (Pcna) and the two migration-related genes - fibroblast growth factor 2 (Fgf2) and delta like non-canonical notch ligand 1 (Dlk1) were also found up-regulated, suggesting that TENG stimulation regulates cell proliferation and migration at the level of gene expression. The present study demonstrates the effectiveness of TENG and its safe operation conditions in biomedical stimulation, which paves a way for practical application of TENG on tissue formation, reepithelialization and tissue remodeling.