Measurement of neuropeptides in clinical samples using chip-based immunoaffinity capillary electrophoresis
Measurement of neuropeptides in clinical samples using chip-based immunoaffinity capillary electrophoresis
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DOI：
10.1016/j.chroma.2006.01.102
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发表时间：
2006-04-07
影响因子：
4.1
通讯作者：
Wellner, E
中科院分区：
文献类型：
--
作者：
Phillips, TM;Wellner, E
目前对微制造的兴趣已经延伸到临床竞技场，其中有越来越多的游说者促进这些用于即时护理目的。这种设备的优点是它们的相对分析速度、较低的试剂成本以及它们在临床筛选和诊断中的应用。两个芯片为基础的毛细管电泳系统已被设计和他们的性能进行评估，快速测量神经肽相关的肌肉疼痛患者的组织液中的炎性神经肽的浓度。两种芯片都被制造成适合市售的芯片电泳系统。一个芯片被设计用于进行电动流动免疫测定，而另一个芯片利用免疫亲和端口，包含一系列固定化抗体，以捕获感兴趣的分析物。结果与市售高灵敏度免疫测定的比较表明，这两种基于芯片的系统可以提供一种相对快速，准确的方法来研究复杂生物液体中的炎症生物标志物。然而，免疫亲和捕获系统证明了这两种芯片的上级。使用该系统，与使用流动免疫测定芯片时的30分钟相比，可以在大约2分钟内确定12种不同的炎症相关介质。随着商业上可获得的抗体阵列的不断扩大，这种基于芯片的系统可以应用于各种不同的分析。(c)2006 Elsevier B.V.保留所有权利。
The current interest in micro-fabrication has extended to the clinical arena where there is a growing lobby for promoting these for point-of-care purposes. The advantages of such devices are their relative speed of analysis, lower reagent costs, and their application to clinical screening and diagnosis. Two chip-based capillary electrophoresis systems have been designed and their performance evaluated for rapidly measuring the concentrations of inflammatory neuropeptides in tissue fluids of patients with neuropeptide-associated muscle pain. Both chips were manufactured to fit a commercially available chip electrophoresis system. One chip was designed to perform electrokinetic flow immunoassays while the other utilized an immunoaffinity port, containing an array of immobilized antibodies, to capture the analytes of interest. Comparison of the results to commercially available high-sensitivity immunoassays demonstrated that both chip-based systems could provide a relatively fast, accurate procedure for studying inflammatory biomarkers in complex biological fluids. However, the immunoaffinity capture system proved the superior of the two chips. Using this system, twelve different inflammation-associated mediators could be determined in approximately 2 min as compared to 30 min when using the flow immunoassay chip. With the ever-expanding array of antibodies that are commercially available, this chip-based system can be applied to a wide variety of different analyses. (c) 2006 Elsevier B.V. All rights reserved.