DOMINANT AWN INHIBITOR Encodes the ALOG Protein Originating from Gene Duplication and Inhibits AWN Elongation by Suppressing Cell Proliferation and Elongation in Sorghum.
DOMINANT AWN INHIBITOR Encodes the ALOG Protein Originating from Gene Duplication and Inhibits AWN Elongation by Suppressing Cell Proliferation and Elongation in Sorghum.
复制标题
显性 AWN 抑制剂编码源自基因复制的 ALOG 蛋白，并通过抑制高粱中的细胞增殖和伸长来抑制 AWN 伸长。
DOI：
10.1093/pcp/pcac057
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发表时间：
2022
影响因子：
4.9
通讯作者：
Wataru Sakamoto
中科院分区：
文献类型：
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作者：
Hideki Takanashi;Hiromi Kajiya-Kanegae;Asuka Nishimura;Junko Yamada;Motoyuki Ishimori;Masaaki Kobayashi;Kentaro Yano;Hiroyoshi Iwata;Nobuhiro Tsutsumi;Wataru Sakamoto
芒是一种针状结构，从禾本科植物的外膜顶端延伸出来，在环境适应和适应方面发挥着作用。在一些作物中，芒似乎在驯化过程中被消除了。虽然已确定了许多与芒发育有关的基因，但也已知存在几个消除芒的显性基因。例如，在高粱(高粱)中，显性的抑芒基因自1921年以来就已被发现，但其分子特征仍未确定。在本研究中，我们对高粱的芒相关性状进行了数量性状座位分析和全基因组关联研究，并确定了编码3号染色体上的ALG家族蛋白的DOMINANT AWN抑制剂(DAI)，DAI似乎存在于大多数无芒高粱品种中，可能是因为它的有效性。对谷物中Alog蛋白家族的详细分析表明，DAI起源于其位于10号染色体上的双胞胎伴生基因(DAIori)的重复。对近等基因系中未成熟芒的观察表明，DAI通过抑制细胞增殖和伸长来抑制芒的伸长。我们还发现，只有DAI通过一种与DAIori不同的芒特异性表达模式获得了抑制芒伸长的新功能。有趣的是，DAI及其自身启动子在水稻中的异源表达抑制了芒品种Kasalath的芒的伸长。我们发现DAI起源于基因复制，提供了一个有趣的功能获得的例子，这种功能只在高粱中发生，但与水稻和高粱共享其功能。
The awn, a needle-like structure extending from the tip of the lemma in grass species, plays a role in environmental adaptation and fitness. In some crops, awns appear to have been eliminated during domestication. Although numerous genes involved in awn development have been identified, several dominant genes that eliminate awns are also known to exist. For example, in sorghum (Sorghum bicolor), the dominant awn-inhibiting gene has been known since 1921; however, its molecular features remain uncharacterized. In this study, we conducted quantitative trait locus analysis and a genome-wide association study of awn-related traits in sorghum and identifiedDOMINANT AWN INHIBITOR(DAI), which encodes the ALOG family protein on chromosome 3.DAIappeared to be present in most awnless sorghum cultivars, likely because of its effectiveness. Detailed analysis of the ALOG protein family in cereals revealed thatDAIoriginated from a duplication of its twin paralog (DAIori) on chromosome 10. Observations of immature awns in near-isogenic lines revealed that DAI inhibits awn elongation by suppressing both cell proliferation and elongation. We also found that onlyDAIgained a novel function to inhibit awn elongation through an awn-specific expression pattern distinct from that ofDAIori. Interestingly, heterologous expression ofDAIwith its own promoter in rice inhibited awn elongation in the awned cultivar Kasalath. We found thatDAIoriginated from gene duplication, providing an interesting example of gain-of-function that occurs only in sorghum but shares its functionality with rice and sorghum.