Petroleum-degrading achlorophyllous alga Prototheca zopfii
Petroleum-degrading achlorophyllous alga Prototheca zopfii
复制标题
石油降解无叶绿藻 Prototheca zopfii
作者：
J. D. Walker;R. Colwell;Z. Vaituzis;S. A. Meyer
该研究计划的目标之一是枚举和鉴定切萨皮克湾中存在的石油降解微生物，评估这些微生物体外和原位降解石油的潜力，并确定降解石油的类型1。我们每月对切萨皮克湾的两个地点进行采样。一个是巴尔的摩港受石油污染的科尔盖特溪，另一个是东湾帕森岛以南的地区，切萨皮克湾的一个未受污染的贝类捕捞区。通过对两个地点收集的沉积物苯提取物进行计算机低分辨率质谱分析，证实了科尔盖特溪中存在石油，而东湾中不存在石油2、3。我们经常从科尔盖特溪和东湾分离出石油降解细菌、酵母和丝状真菌，但在 1973 年 4 月和 5 月以及 1974 年，从科尔盖特溪的沉积物中分离出了一种不寻常的生物体，据信是一种藻类。东湾尚未分离出相应的菌株。
ONE of the objectives of this research programme is to enumerate and identify petroleum-degrading microorganisms occurring in Chesapeake Bay, to assess the potential of these microorganisms to degrade petroleumin vitroandin situand to determine the types of petroleum degraded1. We are sampling two locations in Chesapeake Bay at monthly intervals. One is Colgate Creek, an oil-contaminated site in Baltimore Harbour, the other is an area south of Parson's Island in Eastern Bay, a non-polluted shellfish harvesting region of Chesapeake Bay. The presence of petroleum in Colgate Creek and its absence in Eastern Bay has been confirmed by computerised low-resolution mass spectrometry analysis of benzene extracts of sediments collected at both sites2,3. We have routinely isolated petroleum-degrading bacteria, yeasts and filamentous fungi from Colgate Creek and Eastern Bay, but during April and May 1973, and again in 1974, an unusual organism, believed to be an algal species, was isolated from sediment of Colgate Creek. Corresponding isolates have not been isolated from Eastern Bay.