Application of one‐bead one‐structure approach to identification of nonpeptidic ligands
Application of one‐bead one‐structure approach to identification of nonpeptidic ligands
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应用单珠单结构方法鉴定非肽配体
DOI：
10.1002/ddr.430330211
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发表时间：
1994
影响因子：
3.8
通讯作者：
M. Lebl
中科院分区：
文献类型：
--
作者：
M. Staňková;O. Issakova;N. Sepetov;V. Krchňák;Kit S. Lam;M. Lebl
合成并筛选了由烷基化和酰化氨基酸组成的合成化学文库，以确定与模型靶标链霉亲和素结合的结构。该文库是使用“分割合成”制备的，并通过固相结合测定进行筛选。使用质谱法测定阳性反应化合物的结构。合成了阳性化合物以及各种结构类似物并证实了它们的结合。含有咪唑部分和取代的芳族残基的结构证明了结合。 © 1994 Wiley-Less, Inc.
A synthetic chemical library comprised of alkylated and acylated amino acids was synthesized and screened to determine structures that bind to a model target, streptavidin. The library was prepared using “split synthesis” and screened in a solid phase binding assay. The structure of positively reacting compounds was determined using mass spectroscopy. Positive compounds, together with various structural analogs were synthesized and their binding confirmed. Structures containing both an imidazole moiety and a substituted aromatic residue demonstrated binding. © 1994 Wiley‐Less, Inc.
在随机文库中发现生物活性肽：从树脂珠分阶段正交释放后进行溶液相测试。
DOI：
10.1073/pnas.90.24.11708
发表时间：
1993
影响因子：
11.1
作者：
Salmon,SE;Lam,KS;Lebl,M;Kandola,A;Khattri,PS;Wade,S;Pátek,M;Kocis,P;Krchnák,V;Thorpe,D
通讯作者：
Thorpe,D
影响因子：
56.9
作者：
CHO, CY;MORAN, EJ;SCHULTZ, PG
通讯作者：
SCHULTZ, PG