Exon 2 of human cathepsin B derives from an Alu element
Exon 2 of human cathepsin B derives from an Alu element
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DOI：
10.1016/s0014-5793(97)01445-2
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发表时间：
1997-12-08
期刊：
影响因子：
3.5
通讯作者：
Sloane, BF
中科院分区：
文献类型：
--
作者：
Berquin, IM;Ahram, M;Sloane, BF
半胱氨酸蛋白酶组织蛋白酶B的转录物在非翻译区（UTR）中选择性剪接。我们发现，含有5 '-UTR序列的组织蛋白酶B探针除了与典型的2.2和4.0 kbp的mRNA杂交外，还与约300 nt的RNA杂交。在该5'-UTR中，外显子2与Alu重复元件同源。具体地，外显子2是散布有组织蛋白酶B基因的Alu元件的一部分。与我们的组织蛋白酶B探针杂交的类似于300 nt的条带可能对应于已知在人类细胞中积累的Alu转录物。实际上，用真实的Alu探针检测到类似的迁移带。因此，我们认为组织蛋白酶B的初级转录本含有Alu序列，在一些完全剪接的mRNA中，Alu序列被保留为ex：on 2。(C)1997年欧洲生物化学学会联合会。
Transcripts for the cysteine protease cathepsin B are alternatively spliced in the untranslated regions (UTRs). We show that a cathepsin B probe containing 5'-UTR sequences hybridized to an RNA of similar to 300 nt in addition to the typical 2.2 and 4.0 kbp mRNAs, Within this 5'-UTR, exon 2 was found to be homologous to Alu repetitive elements. Specifically, exon 2 was part of an Alu element interspersed with the cathepsin B gene, The similar to 300 nt band that hybridized to our cathepsin B probe likely corresponds to Alu transcripts, which are known to accumulate in human cells. Indeed, a similarly migrating band was detected with an authentic Alu probe. Thus, we suggest that primary transcripts for cathepsin B contain Alu sequences which are preserved as ex:on 2 in some fully spliced mRNAs. (C) 1997 Federation of European Biochemical Societies.