Incorporation of bioactive polyvinylpyrrolidone-iodine within bilayered collagen scaffolds enhances the differentiation and subchondral osteogenesis of mesenchymal stem cells
Incorporation of bioactive polyvinylpyrrolidone-iodine within bilayered collagen scaffolds enhances the differentiation and subchondral osteogenesis of mesenchymal stem cells
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DOI：
10.1016/j.actbio.2013.05.014
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发表时间：
2013-09-01
影响因子：
9.7
通讯作者：
Ouyang, Hong Wei
中科院分区：
文献类型：
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作者：
Jiang, Yangzi;Chen, Longkun;Ouyang, Hong Wei
聚乙烯吡咯烷酮碘（聚维酮碘，PVP-I）被广泛用作关节手术期间冲洗的防腐剂;然而，PVP-I对关节组织细胞的生物学效应尚不清楚。本研究检测了PVP-I对关节组织细胞的生物相容性和生物学效应，旨在优化基于细胞支架的关节修复。从关节组织中分离细胞，包括软骨来源的祖细胞（CPC）、软骨下骨来源的成骨细胞和骨髓来源的间充质干细胞（BM-MSC）。评价PVP-I对细胞增殖、迁移和分化的浓度依赖性作用。此外，在兔模型中研究了负载PVP-I的双层胶原支架用于骨软骨缺损修复的功效和机制。发现微摩尔浓度的PVP-I不影响细胞增殖、CPC迁移或细胞外基质产生。有趣的是，微摩尔浓度的PVP-I促进BM-MSC的成骨分化，如RUNX 2和骨钙素基因表达的上调以及三维支架上矿化的增加所证明的。PVP-I处理的胶原支架显着增加纤连蛋白结合到支架表面和胶原蛋白I型蛋白的合成培养的BM-MSC。与单独的支架相比，将PVP-I处理的胶原支架植入兔骨软骨缺损中在术后6周显著增强了软骨下骨再生（软骨下骨组织学评分为8.80 +/- 1.64对3.8 +/- 2.19，p < 0.05）。因此，PVP-I对来自关节组织的细胞的生物相容性和促成骨活性以及PVP-I处理的支架中增强的软骨下骨形成将表明PVP-I用于骨软骨缺损修复的潜力。(C)2013 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Polyvinylpyrrolidone-iodine (Povidone-iodine, PVP-I) is widely used as an antiseptic agent for lavation during joint surgery; however, the biological effects of PVP-I on cells from joint tissue are unknown. This study examined the biocompatibility and biological effects of PVP-I on cells from joint tissue, with the aim of optimizing cell-scaffold based joint repair. Cells from joint tissue, including cartilage derived progenitor cells (CPC), subchondral bone derived osteoblast and bone marrow derived mesenchymal stem cells (BM-MSC) were isolated. The concentration-dependent effects of PVP-I on cell proliferation, migration and differentiation were evaluated. Additionally, the efficacy and mechanism of a PVP-I loaded bilayer collagen scaffold for osteochondral defect repair was investigated in a rabbit model. A micromolar concentration of PVP-I was found not to affect cell proliferation, CPC migration or extracellular matrix production. Interestingly, micromolar concentrations of PVP-I promote osteogenic differentiation of BM-MSC, as evidenced by up-regulation of RUNX2 and Osteocalcin gene expression, as well as increased mineralization on the three-dimensional scaffold. PVP-I treatment of collagen scaffolds significantly increased fibronectin binding onto the scaffold surface and collagen type I protein synthesis of cultured BM-MSC. Implantation of PVP-I treated collagen scaffolds into rabbit osteochondral defect significantly enhanced subchondral bone regeneration at 6 weeks post-surgery compared with the scaffold alone (subchondral bone histological score of 8.80 +/- 1.64 vs. 3.8 +/- 2.19, p < 0.05). The biocompatibility and pro-osteogenic activity of PVP-I on the cells from joint tissue and the enhanced subchondral bone formation in PVP-I treated scaffolds would thus indicate the potential of PVP-I for osteochondral defect repair. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.