Effects of temperature on competition and relative dominance of Bradyrhizobium japonicum and Bradyrhizobium elkanii in the process of soybean nodulation
Effects of temperature on competition and relative dominance of Bradyrhizobium japonicum and Bradyrhizobium elkanii in the process of soybean nodulation
复制标题
大豆结瘤过程中温度对日本慢生根瘤菌和艾氏慢生根瘤菌竞争和相对优势度的影响
DOI：
10.1007/s11104-013-1924-5
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发表时间：
2013
期刊：
影响因子：
4.9
通讯作者：
K. Suyama
中科院分区：
文献类型：
--
作者：
Yuta Suzuki;D. Adhikari;K. Itoh;K. Suyama
背景与目的在我们先前的研究中，尼泊尔温带和亚热带地区的大豆根瘤分别由日本慢根瘤菌和伊氏慢根瘤菌引起。本研究旨在揭示温度对细菌结瘤优势的影响。日本和B。Elkani和阐明温度影响与慢生根瘤菌种类分布的气候相关性之间的关系。日本和B。从尼泊尔同一温带地区分离出来的Elkaniistrains。将每株菌的混合物接种到灭菌的含大豆种子或不含大豆种子的蛭石中，接种样品分别在33/27(昼夜)和23/17℃下培养，在接种后0、1、2和4周分别用每株菌的特异性引物竞争PCR检测非根际、根际和根瘤中的相对种群。日本和B。Elkaniistrains在两种温度下都形成了结节。在竞争条件下，低温条件下日本双胞菌占优势，而高温条件下，两个菌株均在1周内达到共结瘤。Elkaniidomin在2周后。在低温条件下，两个菌株在非根际和根际的相对种群相似，但B。在高温条件下，伊尔卡尼斯虫在这些地区占主导地位。低温下根瘤中的日本血吸虫菌株可能是由于优先侵染，而B。高温下的白背飞虱似乎是由于白纹伊蚊种群较多所致。除在共结瘤后在根瘤中占优势外，还可在非根际和根际中生长。温度对白僵菌竞争的影响。日本和B。Elkaniistrains是显着的，并与尼泊尔缓慢的根瘤菌的分布相一致。
Background and aimsBradyrhizobium japonicumandBradyrhizobium elkaniidominated soybean nodules in temperate and subtropical regions in Nepal, respectively, in our previous study. The aims of this study were to reveal the effects of temperature on the nodulation dominancy ofB. japonicumandB. elkaniiand to clarify the relationship between the effects of temperature and the climate-dependent distribution ofBradyrhizobiumspecies.MethodsA laboratory competition experiment was conducted betweenB. japonicumandB. elkaniistrains isolated from the same temperate location in Nepal. A mixture of each strain was inoculated into sterilized vermiculite with or without soybean seeds, and inoculated samples were incubated at 33/27 (day/night) and 23/17 °C. Relative populations in the non-rhizosphere, rhizosphere, and nodules were determined by competitive PCR using specific primers for each strain at 0, 1, 2, and 4 weeks after inoculation.ResultsBoth separately inoculatedB. japonicumandB. elkaniistrains formed nodules at both temperatures. Under competitive conditions,B. japonicumstrains dominated at low temperature; however, at high temperature, both strains achieved co-nodulation in 1 week, withB. elkaniidominating after 2 weeks. The relative populations of both strains were similar in the non-rhizosphere and rhizosphere at low temperature, butB. elkaniistrains dominated in these regions at high temperature.ConclusionsThe domination ofB. japonicumstrains in nodules at the low temperature appeared to be due to preferential infection, while the domination ofB. elkaniistrains at high temperature appeared to be due to the higher population ofB. elkaniiin the non-rhizosphere and rhizosphere, in addition to its domination in nodules after co-nodulation. The effects of temperature on the competition betweenB. japonicumandB. elkaniistrains were remarkable and corresponded with the distribution of bradyrhizobial species in Nepal.