Photosynthetic characterization and expression profiles of sugarcane infected by Sugarcane mosaic virus (SCMV)
Photosynthetic characterization and expression profiles of sugarcane infected by Sugarcane mosaic virus (SCMV)
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DOI：
10.1007/s11120-019-00706-w
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发表时间：
2020-01-03
影响因子：
3.7
通讯作者：
Zhang, Muqing
中科院分区：
文献类型：
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作者：
Akbar, Sehrish;Yao, Wei;Zhang, Muqing
甘蔗花叶病毒属于马铃薯Y病毒科马铃薯Y病毒属，是危害以甘蔗为主的几种重要农作物的重要病原。由于甘蔗本身的复杂性，SCMV致病对甘蔗光合作用系统的影响还有待进一步研究。本研究从细胞病理学、生理生物学、转录组和蛋白质组水平研究了不同甘蔗品种光合作用系统的变化。我们利用Saccharum Sautaneum L获得了两种基因(敏感的Badila和抗性的B-48)的转录组。作为参考基因组。RNA测序结果显示，B-48的NAD(P)H、Rubisco、放氧复合体、叶绿素结合蛋白、PSB蛋白家族、PSI反应中心亚基II和IV基因均显著上调。B-48中上调的基因与光系统的稳定和组装、光抑制的保护和抗病毒防御等多种过程有关。在蛋白质组学水平上进一步验证了差异丰富基因的表达模式。总体而言，差异表达基因/蛋白(DEGS/DEPS)在B-48基因的转录组和蛋白质组水平上的表达是一致的。综上所述，这些数据支持B-48基因在病毒侵染条件下的有效性，并为更好地了解甘蔗光合作用相关基因的表达模式提供了依据。
Sugarcane mosaic virus(SCMV), belonging to genusPotyvirus, familyPotyviridae, is a severe pathogen of several agricultural important crops, mainly sugarcane. Due to complex nature of sugarcane, the effect of SCMV pathogenicity on sugarcane photosynthetic systems remains to be explored. In this study, we investigated the alterations occurring in the photosynthetic system in the sugarcane genotypes at the cytopathological, physiological and biological, transcriptome and proteome level. We generated the transcriptome assembly of two genotypes (susceptible Badila and resistant B-48) usingSaccharum spontaneumL. as a reference genome. RNA-sequencing data revealed the significant upregulation of NAD(P)H, RubisCO, oxygen-evolving complex, chlorophyllaandbbinding protein, Psb protein family, PSI reaction center subunit II, and IVgenes in B-48, as compared to its counterparts. Upregulated genes in B-48 are associated with various processes such as stability and assembly of photosystem, protection against photoinhibition and antiviral defense. The expression pattern of differentially abundant genes were further verified at the proteomics level. Overall, differentially expressed genes/proteins (DEGs/DEPs) showed the consistency of expression at both transcriptome and proteome level in B-48 genotype. Comprehensively, these data supported the efficiency of B-48 genotype under virus infection conditions and provided a better understanding of the expression pattern of photosynthesis-related genes in sugarcane.