CAPILLARY GAS-CHROMATOGRAPHIC PROFILING OF TOTAL LONG-CHAIN FATTY-ACIDS AND CHOLESTEROL IN BIOLOGICAL-MATERIALS
CAPILLARY GAS-CHROMATOGRAPHIC PROFILING OF TOTAL LONG-CHAIN FATTY-ACIDS AND CHOLESTEROL IN BIOLOGICAL-MATERIALS
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DOI：
10.1016/s0378-4347(00)84782-9
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
VANDERSLIK, W
中科院分区：
文献类型：
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作者：
MUSKIET, FAJ;VANDOORMAAL, JJ;VANDERSLIK, W
介绍了毛细管气相色谱-火焰离子化检测法测定多种生物材料中总长链脂肪酸和胆固醇的方法。考察了红细胞样品中15种脂肪酸和胆固醇的批内精密度和日间精密度。给出了30~38周孕妇羊水样本分析的定量数据，并对她们的卵磷脂/鞘磷脂和棕榈酸/硬脂酸比值进行了相关性研究。此外，该方法还应用于腰脑脊液、血浆、分离的白血病血细胞和神经母细胞瘤组织。
The profiling of total long-chain fatty acids and cholesterol in a variety of biological materials, using capillary gas chromatography with flame ionization detection, is described. The within-run precision and day-to-day precision for 15 fatty acids and cholesterol in erythrocyte samples were investigated. Quantitative data on the analysis of amniotic fluid samples collected from women in the 30th to 38th wk are given together with a correlation study on their lecithin/sphingomyelin and their palmitic acid/stearic acid ratios. In addition, the method was applied to lumbar CSF, plasma, isolated leukemic blood cells and neuroblastoma tissue.