CYTOSOLIC CHLORIDE-IONS STIMULATE CA2+-INDUCED EXOCYTOSIS IN MELANOTROPHS
CYTOSOLIC CHLORIDE-IONS STIMULATE CA2+-INDUCED EXOCYTOSIS IN MELANOTROPHS
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DOI：
10.1016/0014-5793(92)80524-k
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发表时间：
1992-06-01
期刊：
影响因子：
3.5
通讯作者：
ZOREC, R
中科院分区：
文献类型：
--
作者：
RUPNIK, M;ZOREC, R
我们使用全细胞膜片钳技术通过监测膜电容的变化来研究单细胞的分泌活性[Neher， E.和Marty， A. (1982) Proc. Natl.]。学会科学。[美国文献79,523-535]。出乎意料的是，我们观察到增加的细胞内氯离子以剂量依赖性的方式刺激Ca2+诱导的胞外分泌（K(d) = 12 mM）。这些结果证明了胞质氯离子在调节垂体前叶细胞胞外分泌中的作用。这表明氯离子通道除了在调节膜电活动中起作用外[Korn， s.j., Bolden]。王晓明，王晓明(1);[j] .中国生物医学工程学报。R.彭纳，马修斯。王晓明，王晓明，王晓明，等（1988）细胞内pH值的测定[j]。也参与胞质氯离子浓度的调节，从而控制胞吐。
We used thc whole-cell patch-clamp technique to study the secretory activity of single cells by monitoring changes in membrane capacitance [Neher, E. and Marty, A. (1982) Proc. Natl. Acad. Sci. USA 79, 523-535] in anterior pituitary cells. Unexpectedly we have observed that increasing intracellular chloride ions stimulate Ca2+-induced exocytosis in a dose-dependent fashion (K(d) = 12 mM). These results demonstrate a role of cytosolic chloride ions in the regulation of exocytotic secretion in anterior pituitary cells. It is suggested that chloride channels, in addition to playing a part in regulating membrane electrical activity [Korn, S.J., Bolden. A. and Horn, A. (I 991) J. Physiol. 439, 423-437; Penner, R., Matthews. G. and Horn, A. (1988) Nature 334, 499-504] and cytosolic pH [Kaila, K. and Voipio, J. (1987) Nature 330, 163-165]. are also involved in the modulation of cytosolic chloride concentration and thus in the control of exocytosis.