Embryo developmental capability and pregnancy outcome are related to the mitochondrial DNA copy number and ooplasmic volume
Embryo developmental capability and pregnancy outcome are related to the mitochondrial DNA copy number and ooplasmic volume
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DOI：
10.1007/s10815-013-0062-6
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发表时间：
2013-10-01
影响因子：
3.1
通讯作者：
Yoshimura, Yasunori
中科院分区：
文献类型：
--
作者：
Murakoshi, Yukitaka;Sueoka, Kou;Yoshimura, Yasunori
探讨胚胎卵浆体积与线粒体DNA （mtDNA）拷贝数的关系及其对生殖力的影响。利用实时荧光定量PCR技术，对未受精卵母细胞和未卵裂胚胎进行mtDNA定量分析。通过计算体外受精（IVF）或胞浆内单胞注射（ICSI）去除颗粒细胞时的卵浆体积来评估卵子的大小。取卵后72h，裂胚7-8细胞期，采用实时荧光定量PCR对卵裂球mtDNA进行定量分析。我们计算了卵裂球的细胞质体积。我们的研究显示，年龄大于40岁的女性未受精卵母细胞和未卵裂胚胎的mtDNA拷贝数显著降低（p < 0.05）。卵浆体积越大，卵裂越早、越快（p < 0.05）。怀孕组的卵浆体积也明显增大。我们发现卵裂球体积与mtDNA拷贝数呈正相关（r = 0.76, p < 0.01，来自Pearson积差相关系数）。我们已经证明，卵裂球体积与mtDNA拷贝数成正比。因此，更大的细胞质体积，更早的分裂速度，意味着更多的mtDNA拷贝。评价mtDNA的定量和卵母浆和卵裂球体积的测定可能有助于选择高质量的胚胎和妊娠结局。
To investigate the correlation between the ooplasmic volume and the number of mitochondrial DNA (mtDNA) copies in embryos and how they may affect fecundity.Using real-time PCR, mtDNA quantification was analyzed in unfertilized oocytes and uncleaved embryos. The size of the ovum was also assessed by calculating the ooplasmic volume at the time of granulosa cell removal for IVF or ICSI. Quantification analysis of the mtDNA in blastomeres was performed by real-time PCR at the 7-8 cell stage of the cleaved embryos at 72 h after oocyte retrieval. We calculated the cytoplasmic volume of the blastomeres.Our studies showed a significantly lower mtDNA copy number in unfertilized oocytes and uncleaved embryos in women who were older than 40 years of age (p < 0.05). The larger ooplasmic volume was also associated with earlier and more rapid cleavage (p < 0.05). The ooplasmic volume was also significantly larger in the group achieving pregnancy. We found a significant positive correlation between blastomere volume and the number of mtDNA copies (r = 0.76, p < 0.01, from Pearson product-moment correlation coefficient).We have shown that blastomere volume is directly proportional to the number of mtDNA copies. Therefore, larger cytoplasmic volume, with earlier cleavage speed, implies more mtDNA copies. Evaluation of mtDNA quantification and the measurement of ooplasmic and blastomere volume may be useful for selection of high quality embryo and pregnancy outcome.