Myosin heavy-chain-based isomyosins in developing, adult fast-twitch and slow-twitch muscles.
Myosin heavy-chain-based isomyosins in developing, adult fast-twitch and slow-twitch muscles.
复制标题
发育中的成人快肌和慢肌中基于肌球蛋白重链的同肌球蛋白。
DOI：
10.1111/j.1432-1033.1991.tb15740.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
D. Pette
中科院分区：
文献类型：
--
作者：
A. Termin;D. Pette
非变性条件下电泳的改良方法使得有可能分离大鼠肌肉提取物的定义肌球蛋白重链（HC）和轻链（LC）的组合物到子集的发展，快速和慢速肌球蛋白重链为基础的isomyosins。迁移最快的异肌球蛋白是新生异肌球蛋白（nM 1，nM 2，nM 3），其次是迁移稍慢的胚胎异肌球蛋白（eM 1，eM 2，eM 3，eM 4）。在9种成人快速异肌球蛋白中，以HCIIb为基础的异肌球蛋白（FM 1b，FM 2b，FM 3b）迁移最快。其次是基于HCIId的异肌球蛋白（FM 1d、FM 2d、FM 3d）。以HCIIa为基础的异肌球蛋白（FM 1a，FM 2a，FM 3a）的反应速度最慢。我们的研究结果表明，FM 3a与文献中描述的所谓中间体异肌球蛋白（IM）相同。慢肌球蛋白重链为基础的异肌球蛋白（SM 1，SM 2，SM 3）迁移远远落后于快速异肌球蛋白。尽管这些异肌球蛋白三联体中的每一个的总电泳迁移率由特定的重链互补决定，但是每个三联体内的条带的不同迁移率由不同的碱性轻链组合产生。因此，新生儿（nM 1）和成人快速异肌球蛋白（FM 1b，FM 1d，FM 1a）的最快三重带代表LC 3f同源二聚体，最慢的（nM 3，FM 3b，FM 3d，FM 3a）代表LC 1f同源二聚体，中间带（nM 2，FM 2b，FM 2d，FM 2a）代表LC 1f/LC 3f异源二聚体。不同比例的成人快速异肌球蛋白三联体条带表明，对LC 3f的亲和力按HCIIb，HCIId，HCIIa的顺序降低。三种慢肌球蛋白代表LC 1 sa（SM 1）和LC 1 sb（SM 3）同源二聚体以及LC 1 sa/LC 1 sb异源二聚体（SM 2）。间接证据表明，在兔肌肉中，SM 1和SM 3最有可能分别代表LC 1 sb和LC 1 sa同源二聚体，顺序相反。
A modified method of electrophoresis under nondenaturing conditions made it possible to separate rat muscle extracts of defined myosin heavy chain (HC) and light chain (LC) composition into subsets of developmental, fast and slow myosin heavy-chain-based isomyosins. The fastest migrating isomyosins were the neonatal isomyosins (nM1, nM2, nM3), followed by the slightly slower migrating embryonic isomyosins (eM1, eM2, eM3, eM4). Of the nine adult fast isomyosins, the HCIIb-based isomyosins (FM1b, FM2b, FM3b) were the fastest migrating. These were followed by the HCIId-based isomyosins (FM1d, FM2d, FM3d). The HCIIa-based isomyosins (FM1a, FM2a, FM3a) were the slowest. Our results suggest that FM3a is identical with the so-called intermediate isomyosin (IM) described in the literature. The slow myosin heavy-chain-based isomyosins (SM1, SM2, SM3) migrated far behind the fast isomyosins. Whereas the gross electrophoretic mobilities of each of these isomyosin triplets is determined by the specific heavy chain complement, the different mobilities of the bands within each triplet result from different alkali light chain combinations. Thus, the fastest triplet bands of the neonatal (nM1) and adult fast isomyosins (FM1b, FM1d, FM1a) represent the LC3f homodimers, the slowest (nM3, FM3b, FM3d, FM3a) the LC1f homodimers, and the intermediate bands (nM2, FM2b, FM2d, FM2a) the LC1f/LC3f heterodimers. Different proportions of the adult fast isomyosin triplet bands indicate that the affinity for LC3f decreases in the order HCIIb, HCIId, HCIIa. The three slow isomyosins represent LC1sa (SM1) and LC1sb (SM3) homodimers and a LC1sa/LC1sb heterodimer (SM2). Circumstantial evidence suggests an inverse order in rabbit muscle where SM1 and SM3 most likely represent LC1sb and LC1sa homodimers, respectively.
DOI：
10.1016/s0021-9258(18)77444-9
发表时间：
1990-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Kirschbaum;H. Kucher;A. Termin;A. Kelly;D. Pette
通讯作者：
B. Kirschbaum;H. Kucher;A. Termin;A. Kelly;D. Pette
啮齿动物同肌球蛋白的亚基组成及其在后肢骨骼肌中的分布。
DOI：
10.1152/jappl.1987.63.5.2101
发表时间：
1987
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Tsika,RW;Herrick,RE;Baldwin,KM
通讯作者：
Baldwin,KM
骨骼肌肌球蛋白同工酶随肥大和运动的变化。
DOI：
10.1042/bj2380055
发表时间：
1986
期刊：
The Biochemical journal
影响因子：
--
作者：
Gregory,P;Low,RB;Stirewalt,WS
通讯作者：
Stirewalt,WS