Bioanalytical Technologies for Safety Control of Fish and Seafood by Sensitive Rapid Tests for Phycotoxins
Bioanalytical Technologies for Safety Control of Fish and Seafood by Sensitive Rapid Tests for Phycotoxins
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通过藻毒素的灵敏快速检测来安全控制鱼类和海鲜的生物分析技术
DOI：
10.1088/1755-1315/988/4/042041
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
A. Hibara
中科院分区：
文献类型：
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作者：
S. A. Eremin;A. V. Zherdev;M. Tokeshi;A. Hibara
现代社会对食品安全监测的要求越来越高。这项工作旨在开发和表征新的测试系统，以控制藻毒素，鱼类和海产品的危险污染物。为了快速高效的检测，有两种方法：免疫层析法和荧光偏振免疫法。考虑了降低目标分析物检测限的各种方法，包括使用光学和荧光标记的变化以及检测到的络合物形成的不同顺序。抗体和适体被认为是受体分子。开发的技术提供快速（20-30分钟，包括样品制备）和敏感的测试。它们对不同种类的鱼和海鲜都有效。便携式检测器已经提出，允许在采样点直接检测，而不需要运输到集中实验室。
Wide and operate safety monitoring of foodstuffs is highly demanded in modern society. This work aims to develop and characterize new test systems for control of phycotoxins, dangerous contaminants of fish and seafood. For rapid and productive testing, 2 methods have been implemented: immunochromatographic assay and fluorescence polarization immunoassay. Various approaches for reducing the limit of detection of target analytes have been considered, including changes in the use of optical and fluorescent labels and varied order of the detected complexes formation. Antibodies and aptamers have been considered as receptor molecules. The developed techniques provide rapid (20–30 minutes, including sample preparation) and sensitive testing. Their effectiveness has been shown for different kinds of fish and seafood. Portable detectors have been proposed that allow testing directly at the sampling points, without transportation to centralized laboratories.
食品安全保障和兽医公共卫生
DOI：
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发表时间：
2009
期刊：
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影响因子：
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作者：
A. Butterworth
通讯作者：
A. Butterworth