Neural expression profile of α-synuclein in developing human cortex
Neural expression profile of α-synuclein in developing human cortex
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DOI：
10.1097/00001756-199909090-00019
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发表时间：
1999-09-09
期刊：
影响因子：
1.7
通讯作者：
Beyreuther, K
中科院分区：
文献类型：
--
作者：
Bayer, TA;Jäkälä, P;Beyreuther, K
α-突触核蛋白是一种以神经元为主的突触前蛋白，可能在突触发生和中枢神经系统发育过程中发挥重要作用。为了阐明人类的发育表达谱，我们使用了一个多克隆。抗α-突触核蛋白NAC结构域的抗血清。在正常胎儿皮质中，神经外胚层前体细胞在胞体中被染色，而在成人皮质中，我们观察到与突触功能相一致的染色模式。在神经退行性疾病中也发现了相同的发育性神经元内重新分布，即胎儿(21三体)神经外胚层前体细胞的体细胞染色，以及成人(DO NN综合征，阿尔茨海默病)脑中的突触模式。RT-PCR和Western印迹分析显示，在人脑发育过程中的所有时间点(4-7.5月)都有表达。(C)1999年，Lippincott Williams&Wilkins。
alpha-SYNUCLEIN is a predominantly neuronal presynaptic protein that may play an important role during synaptogenesis and CNS development. In order to elucidate the human developmental expression profile, we used a polyclonal. antiserum against the NAC domain of alpha-synuclein. In normal fetal cortex neuroectodermal precursor cells elicited staining in the soma, whereas, in adult cortex, we observed a staining pattern compatible with synaptic function. The same developmental intraneuronal redistribution was found in neurodegenerative disorders, i.e. somatic staining in neuroectodermal precursors in fetal (trisomy 21) and a synaptic pattern in adult (Do Nn's syndrome, Alzheimer's disease) brains. RT-PCR and Western blot analysis revealed expression at all time points studied (4-7.5 months) during human brain development. (C) 1999 Lippincott Williams & Wilkins.