Expression of P-selectin at low site density promotes selective attachment of eosinophils over neutrophils
Expression of P-selectin at low site density promotes selective attachment of eosinophils over neutrophils
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DOI：
10.4049/jimmunol.165.1.404
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发表时间：
2000-07-01
影响因子：
4.4
通讯作者：
Sklar, LA
中科院分区：
文献类型：
--
作者：
Edwards, BS;Curry, MS;Sklar, LA
中性粒细胞与E-选择素和嗜酸性粒细胞与P-选择素的选择性相互作用先前已被报道，但选择素位点密度和流体剪切的相关性尚未详细研究。我们开发了一种新方法来检查细胞悬浮液中的这些相互作用，该方法将细锥板粘度计与流式细胞仪集成在一起，我们发现嗜酸性粒细胞和中性粒细胞都使用 P-选择素糖蛋白配体-1 与 P-选择素中国仓鼠卵巢细胞转染子形成稳定的缀合物，并优先粘附嗜酸性粒细胞。此外，中性粒细胞和嗜酸性粒细胞之间的细胞粘附差异在 P-选择素表达水平低于约 20 个位点/μ m(2) 时被放大，该范围可能与嗜酸性粒细胞增多相关条件下的内皮细胞表达水平相关。在 100 至 150/s 的剪切速率范围内保留了独特的行为，但在低剪切下被放大。平行板流动室测定的结果表明，嗜酸性粒细胞的优先粘附反映了最初 PSGL-1 与 P-选择素形成键的效率提高，而不是嗜酸性粒细胞在低密度 P-选择素底物上介导较长持续时间的滚动相互作用的独特能力。这些差异可能部分解释了过敏性疾病中嗜酸性粒细胞积累的增加。
The selective interaction of neutrophils with E-selectin and eosinophils with P-selectin has been previously reported, but the relevance of selectin site density and fluid shear has not been studied in detail. We have developed a new approach to examine these interactions in cell suspensions that integrates an on-fine cone-plate viscometer with a how cytometer, We End that eosinophils and neutrophils both use P-selectin glycoprotein ligand-1 to form stable conjugates with P-selectin Chinese hamster ovary cell transfectants, with a preferential adhesion of eosinophils. Further, the difference in cell adhesion between neutrophils and eosinophils is magnified at P-selectin expression levels below similar to 20 sites/mu m(2), a range likely to be relevant to endothelial cell expression levels in conditions associated with eosinophilia, The unique behavior is retained over shear rates ranging from 100 to 150/s but is magnified at low shear. Results from parallel-plate flow chamber assays suggest that preferential eosinophil adhesion reflects an enhanced efficiency of initial PSGL-1 bond formation with P-selectin rather than a unique ability of eosinophils to mediate rolling interactions of longer duration on low-density P-selectin substrates, These differences may account in part for the increase in eosinophil accumulation in allergic diseases.