A BLOCKING MONOCLONAL-ANTIBODY TO ENDOTHELIAL-LEUKOCYTE ADHESION MOLECULE-1 (ELAM1)
A BLOCKING MONOCLONAL-ANTIBODY TO ENDOTHELIAL-LEUKOCYTE ADHESION MOLECULE-1 (ELAM1)
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DOI：
10.1016/0006-291x(90)91400-m
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发表时间：
1990-08-31
影响因子：
3.1
通讯作者：
LOBB, R
中科院分区：
文献类型：
--
作者：
BENJAMIN, C;DOUGAS, I;LOBB, R
ELAM 1是一种由炎性细胞因子诱导的人脐静脉内皮细胞（HUVECs）上的白细胞粘附分子。用瞬时表达ELAM 1的COS细胞免疫Balb/C小鼠。融合后，ELAM 1特异性单克隆抗体（单克隆抗体）被确定为选择性粘附ELAM 1表达，但不控制，CHO细胞，并以奎宁处理，但不未经处理的HUVEC。一种称为BB 11的单克隆抗体结合并免疫沉淀在HUVEC、COS和CHO细胞上表达的ELAM 1。BB 11阻断ELAM 1与人PMN、人骨髓单核细胞系HL 60和人结肠癌系HT 29的相互作用。
ELAM1 is a leukocyte adhesion molecule induced on human umbilical vein endothelial cells (HUVECs) by inflammatory cytokines. Balb/C mice were immunized with COS cells transiently expressing cell-surface ELAM1 after transfection with ELAM1 cDNA. After fusion, ELAM1-specific monoclonal antibodies (Mabs) were identified by selective adhesion to ELAM1-expressing, but not control, CHO cells, and to cytokine-treated but not untreated HUVECs. One Mab, designated BB11, binds to and immunoprecipitates ELAM1 expressed on HUVECs, COS and CHO cells. BB11 blocks the interaction of ELAM1 with human PMN, the human myelomonocytic cell line HL60, and the human colon carcinoma line HT29.