Distribution of luteinizing hormone-releasing hormone in the nervus terminalis and brain of the mouse detected by immunocytochemistry.
Distribution of luteinizing hormone-releasing hormone in the nervus terminalis and brain of the mouse detected by immunocytochemistry.
复制标题
免疫细胞化学检测小鼠终神经和大脑中黄体生成素释放激素的分布。
DOI：
10.1002/cne.902550207
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Pfaff,DW
中科院分区：
文献类型：
--
作者：
Schwanzel-Fukuda,M;Garcia,MS;Morrell,JI;Pfaff,DW
免疫反应性促黄体生成素释放激素（LHRH）定位于新生和成年小鼠终神经的相对大量的神经节细胞和纤维中，表明该神经是小鼠脑中LHRH的重要来源。新生小鼠的全头标本，在颅骨钙化之前，显示LHRH神经元和细纤维广泛分布于整个外周、颅内、外胚层和内胚层，和终神经的中央部分。新生动物和成年动物之间最显著的差异是终神经的免疫反应性轴突的增加，这些轴突组成了终神经的纤维束。在成年小鼠中，颅内和中央投射由免疫反应性轴突的厚束组成，被胶质细胞包裹，并伴有含有LHRH反应性和非免疫反应性神经元的神经节。LHRH免疫反应阳性细胞和轴突见于终神经的分支中，该分支沿着嗅球的内侧、后背侧行进，并见于该神经的分支中，该分支伴随犁鼻神经到达副嗅球。在副嗅球和主嗅球中可见少量LHRH神经元和许多免疫反应性突起，在下丘脑和下丘脑外结构中可见LHRH反应性神经元。成年小鼠大脑的检查显示，免疫反应性神经元的分布和数量的模式类似于在新生儿中看到的。然而，在成熟动物大脑的所有区域都看到了更多的LHRH反应性轴突。
Immunoreactive luteinizing hormone‐releasing hormone (LHRH) was localized in a relatively large number of ganglion cells and fibers of the nervus terminalis of neonatal and adult mice, indicating that this nerve is a substantial source of LHRH in the mouse brain.Whole‐head specimens of neonatal mice, prior to calcification of the cranium, revealed an extensive distribution of LHRH neurons and fine fibers throughout the peripheral, intracranial, and central parts of the nervus terminalis. The most striking difference between the neonatal and adult animals, in the nervus terminalis, was the increase in immunoreactive axons that made up the fiber bundles of this nerve. In the adult mouse, the intracranial and central projections were composed of thick fascicles of immunoreactive axons, ensheathed by glial cells and accompanied by ganglia that contained both LHRH‐reactive and nonimmunoreactive neurons. LHRH‐immunoreactive cells and axons were seen in a branch of the nervus terminalis that coursed along the medial, posterodorsal aspect of the olfactory bulb and in branches of this nerve that accompany the vomeronasal nerves to the accessory olfactory bulb. A few LHRH neurons and many immunoreactive processes were seen in the accessory and main olfactory bulbs.LHRH‐reactive neurons were seen in the hypothalamus and extrahypothalamic structures. Examination of adult mouse brains revealed a pattern of distribution and number of immunoreactive neurons similar to that seen in the neonate. However, many more LHRH‐reactive axons were seen in all areas of the brain of the mature animal.