The macrophage capacity for phagocytosis.
The macrophage capacity for phagocytosis.
复制标题
DOI：
--
复制
发表时间：
1992-04
影响因子：
4
通讯作者：
Gregory J. Cannon;Joel A. Swanson
中科院分区：
文献类型：
--
作者：
Gregory J. Cannon;Joel A. Swanson
在悬浮液中测量直径为13.8 +/-2.3微米的鼠骨髓来源的巨噬细胞可以摄取直径大于20微米的IgG调理的乳胶珠。精确的测定可以确定吞噬能力以及限制该能力的生理参数。大于15微米直径的珠粒的摄入需要IgG调理作用，并且需要30分钟才能完成。尽管依赖于Fc受体来吞噬较大的珠粒，但细胞在所有细胞表面Fc受体被占据之前达到其极限。在调理的盖玻片的吞噬作用受挫后的最大膜表面积类似于在极限直径下吞噬颗粒所需的膜表面积，表明该能力与颗粒形状无关。用蔗糖酸化溶酶体室，扩大内吞室，降低吞噬能力。当蔗糖液泡被转化酶孵育的细胞崩溃时，这种下降被逆转。这些实验表明，吞噬能力受到可用膜的量的限制，而不是Fc受体的可用性。诺考达唑使细胞质微管解聚也降低了这种能力。诺考达唑并不影响最大的细胞扩散在吞噬功能受挫的面积，但确实改变了扩散细胞的形状。因此，微管可以协调细胞质吞噬最大的颗粒。
Murine bone marrow-derived macrophages, which measure 13.8 +/- 2.3 microns diameter in suspension, can ingest IgG-opsonized latex beads greater than 20 microns diameter. A precise assay has allowed the determination of the phagocytic capacity, and of physiological parameters that limit that capacity. Ingestion of beads larger than 15 microns diameter required IgG-opsonization, and took 30 minutes to reach completion. Despite the dependence on Fc-receptors for phagocytosis of larger beads, cells reached their limit before all cell surface Fc-receptors were occupied. The maximal membrane surface area after frustrated phagocytosis of opsonized coverslips was similar to the membrane surface area required to engulf particles at the limiting diameter, indicating that the capacity was independent of particle shape. Vacuolation of the lysosomal compartment with sucrose, which expanded endocytic compartments, lowered the phagocytic capacity. This decrease was reversed when sucrose vacuoles were collapsed by incubation of cells with invertase. These experiments indicate that the phagocytic capacity is limited by the amount of available membrane, rather than by the availability of Fc-receptors. The capacity was also reduced by depolymerization of cytoplasmic microtubules with nocodazole. Nocodazole did not affect the area of maximal cell spreading during frustrated phagocytosis, but did alter the shape of the spread cells. Thus, microtubules may coordinate cytoplasm for engulfment of the largest particles.