Passage through stationary phase advances replicative aging in Saccharomyces cerevisiae
Passage through stationary phase advances replicative aging in Saccharomyces cerevisiae
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DOI：
10.1073/pnas.96.16.9100
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发表时间：
1999-08-03
影响因子：
11.1
通讯作者：
Guarente, L
中科院分区：
文献类型：
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作者：
Ashrafi, K;Sinclair, D;Guarente, L
酿酒酵母母细胞经历老化程序，包括形态变化、不育、Sir转录沉默复合物从Hill位点和端粒到核仁的重新分布、核仁结构的改变以及染色体外核糖体DNA环(ERC)的积累。我们在这里报告，在稳定期长时间缺乏营养的细胞在重新进入细胞周期时表现出世代寿命的缩短，这种缩短的寿命不会传递给后代细胞，表明这不是由于不可逆的遗传损伤造成的，寿命的缩短伴随着加速衰老的所有变化，但值得注意的例外是，与世代匹配的非饥饿细胞相比，ERC积累没有增加。这些结果提出了多种模型，其中包括饥饿细胞揭示了衰老的一个组成部分与 ERC 的积累同时起作用，静止期酵母细胞可能是一个有用的系统，用于识别影响其他非分裂真核细胞衰老的因素。
Saccharomyces cerevisiae mother cells undergo an aging program that includes morphologic changes, sterility, redistribution of the Sir transcriptional silencing complex from Hill loci and telomeres to the nucleolus, alterations in nucleolar architecture, and accumulation of extrachromosomaI ribosomal DNA circles (ERCs). We report here that cells starved for nutrients during prolonged periods in stationary phase show a decrease in generational lifespan when they reenter the cell cycle, This shortened lifespan is not transmitted to progeny cells, indicating that it is not due to irreversible genetic damage, The decrease in the lifespan is accompanied by all of the changes of accelerated aging with the notable exception that ERC accumulation is not augmented compared with generation-matched, nonstarved cells, These results suggest a number of models, including one in which starvation reveals a component of aging that works in parallel with the accumulation of ERCs, Stationary-phase yeast cells may be a useful system for identifying factors that affect aging in other nondividing eukaryotic cells.