Hyperactivated motility induced in mouse sperm by calcium ionophore A23187 is reversible.
Hyperactivated motility induced in mouse sperm by calcium ionophore A23187 is reversible.
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DOI：
10.1002/jez.1402440218
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发表时间：
1987-11
期刊：
影响因子：
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通讯作者：
Susan S. Suarez;Leila Vincenti;Matthew W. Ceglia
中科院分区：
文献类型：
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作者：
Susan S. Suarez;Leila Vincenti;Matthew W. Ceglia
用二甲亚砜（DMSO）中1微米钙离子离子A23187处理尾侧附睾小鼠精子，2分钟后加入含有高水平牛血清白蛋白（BSA）的培养基（终浓度：0.5微米A23187, 22 mg/ml BSA），测试其高激活运动的可逆性。对照组仅给予二甲基亚砜，随后给予牛血清白蛋白。立即用A23187治疗后，运动弱且振动。在加入高水平的牛血清白蛋白两分钟后，运动被过度激活，通过录像分析轨迹的线性度和鞭毛弯曲的尖锐度来确定。加药10分钟后，精子的运动模式恢复到新鲜的、无能力的附睾精子的运动模式。在10分钟的观察中，对照精子保持了新鲜尾附睾精子的线性游动模式。然而，90分钟后，对照组和实验组的精子都变得过度活跃。活动精子的百分比不受治疗或时间的影响。因此，离子载体诱导的过度激活是可逆的，并且在体外培养过程中不干扰过度激活的正常发展。
The reversibility of hyperactivated motility was tested in caudal epididymal mouse sperm by treating them with 1 microM calcium ionophore A23187 in dimethyl sulfoxide (DMSO), followed 2 min later by the addition of medium containing high levels of bovine serum albumin (BSA) (final concentrations: 0.5 microM A23187, 22 mg/ml BSA). Controls received DMSO alone, followed by BSA. Immediately following treatment with A23187, motility was weak and vibratory. Two minutes after the addition of high levels of BSA, motility was hyperactivated, as determined by videotape analysis of linearity of trajectory and acuteness of flagellar bending. Ten minutes after the addition, the movement pattern returned to that of fresh, uncapacitated epididymal sperm. Control sperm retained the linear swimming pattern of fresh caudal epididymal sperm during the 10 min of observation. Ninety minutes later, however, both control and treated sperm became hyperactivated. The percentage of motile sperm was not affected by treatment or time. Thus, ionophore-induced hyperactivation is reversible and does not interfere with the normal development of hyperactivation during incubation under capacitating conditions in vitro.