Protective prospects of eco-friendly synthesized selenium nanoparticles using Moringa oleifera or Moringa oleifera leaf extract against melamine induced nephrotoxicity in male rats
Protective prospects of eco-friendly synthesized selenium nanoparticles using Moringa oleifera or Moringa oleifera leaf extract against melamine induced nephrotoxicity in male rats
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DOI：
10.1016/j.ecoenv.2021.112424
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发表时间：
2021-06-23
影响因子：
6.8
通讯作者：
Khalil, Amany T.
中科院分区：
文献类型：
--
作者：
Abu-Zeid, Ehsan H.;Fattah, Doaaa M. Abdel;Khalil, Amany T.
纳米技术应用广泛，包括精确针对疾病预防和治疗的医学疗法。本研究首先以辣木叶提取物（MOLE）为原料，以环境友好的方式合成硒纳米颗粒（SeNPs）。其次，比较绿色合成的MOLE-SeNPs偶联物和MOLE乙醇提取物对三聚氰胺（MEL）所致雄性大鼠肾毒性的保护作用。将105只雄性Sprague道利大鼠分为7组（n = 15），包括第1对照组、第2 MOLE组（800 mg/kg BW）、第3 SeNPs组（0.5 mg/kg BW）、第4 MOLE-SeNPs组（200 μ g/kg BW）、第5 MEL组（700 mg/kg BW）、第6 MEL+MOLE组和第7 MEL+ MOLESeNPs组。所有组均连续28天经口灌胃。通过TEM、SEM和DLS粒径表征SeNPs和胶体SeNPs。SeNPs在530 nm波长处显示出吸收峰，呈球形，平均尺寸在3.2和20 nm之间。在400和700 nm之间记录胶体SeNP吸收光谱，平均尺寸为3.3-17 nm。MEL诱导的肾病改变表现为血清肌酐、尿素、血尿素氮（BUN）、肾TNF α、氧化应激相关指数的显著增加，并改变了凋亡相关基因Bax、Caspase-3、Bcl 2、Fas和FasL的相对mRNA表达。MEL诱导的一系列肾毒性形态学变化，以及增殖细胞核抗原（PCNA）和增殖相关核抗原Ki-67的免疫表达上调。MOLE或MOLE-SeNPs的管理显着逆转MEL诱导的肾功能损害，氧化应激，组织学改变，在相对mRNA表达的糖尿病相关基因的调制，和肾PCNA和Ki-67的免疫表达。总之，绿色合成的MOLE-SeNPs和MOLE显示针对MEL诱导的小鼠肾病的肾单位保护特性。该研究首次报道了这些效应，其在MOLE组中比在绿色生物合成的MOLE-SeNP缀合物组中更明显。
Nanotechnology is used in a wide range of applications, including medical therapies that precisely target disease prevention and treatment. The current study aimed firstly, to synthesize selenium nanoparticles (SeNPs) in an eco-friendly manner using Moringa oleifera leaf extract (MOLE). Secondly, to compare the protective effects of green-synthesized MOLE-SeNPs conjugate and MOLE ethanolic extract as remedies for melamine (MEL) induced nephrotoxicity in male rats. One hundred and five male Sprague Dawley rats were divided into seven groups (n = 15), including 1st control, 2nd MOLE (800 mg/kg BW), 3rd SeNPs (0.5 mg/kg BW), 4th MOLE-SeNPs (200 mu g/kg BW), 5th MEL (700 mg/kg BW), 6th MEL+MOLE, and 7th MEL+MOLE SeNPs. All groups were orally gavaged day after day for 28 days. SeNPs and the colloidal SeNPs were characterized by TEM, SEM, and DLS particle size. SeNPs showed an absorption peak at a wavelength of 530 nm, spherical shape, and an average size between 3.2 and 20 nm. Colloidal SeNPs absorption spectra were recorded between 400 and 700 nm with an average size of 3.3-17 nm. MEL-induced nephropathic alterations represented by a significant increase in serum creatinine, urea, blood urea nitrogen (BUN), renal TNF alpha, oxidative stress-related indices, and altered the relative mRNA expression of apoptosis-related genes Bax, Caspase-3, Bcl2, Fas, and FasL. MEL-induced array of nephrotoxic morphological changes, and up-regulated immune-expression of proliferating cell nuclear antigen (PCNA) and proliferation-associated nuclear antigen Ki-67. Administration of MOLE or MOLE-SeNPs significantly reversed MEL-induced renal function impairments, oxidative stress, histological alterations, modulation in the relative mRNA expression of apoptosis-related genes, and the immune-expression of renal PCNA and Ki-67. Conclusively, the green-synthesized MOLE-SeNPs and MOLE display nephron-protective properties against MEL-induced murine nephropathy. This study is the first to report these effects which were more pronounced in the MOLE group than the green biosynthesized MOLE-SeNPs conjugate group.