Predicting the receptive range of olfactory receptors
Predicting the receptive range of olfactory receptors
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DOI：
10.1371/journal.pcbi.0040018
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发表时间：
2008-02-01
影响因子：
4.3
通讯作者：
Harel, David
中科院分区：
文献类型：
--
作者：
Haddad, Rafi;Carmel, Liran;Harel, David
虽然编码嗅觉受体的基因家族在15年前就被鉴定出来，但是在异源系统中功能性表达这些受体的困难，除了一些例外，使得给定嗅觉受体的接受范围在很大程度上是未知的。此外，即使成功表达，用数千种气味/配体探测这种受体的任务仍然令人生畏。在这里，我们提供了解决这个问题的概念证明。利用计算方法，我们将电子鼻调整到嗅觉受体的接收范围。然后，我们使用这种电子鼻来预测受体对其他气味的反应。我们的方法可用于识别嗅觉受体的接受范围，也可应用于其他问题，涉及受体-配体相互作用在非嗅觉设置。
Although the family of genes encoding for olfactory receptors was identified more than 15 years ago, the difficulty of functionally expressing these receptors in an heterologous system has, with only some exceptions, rendered the receptive range of given olfactory receptors largely unknown. Furthermore, even when successfully expressed, the task of probing such a receptor with thousands of odors/ligands remains daunting. Here we provide proof of concept for a solution to this problem. Using computational methods, we tune an electronic nose to the receptive range of an olfactory receptor. We then use this electronic nose to predict the receptors' response to other odorants. Our method can be used to identify the receptive range of olfactory receptors, and can also be applied to other questions involving receptor-ligand interactions in non-olfactory settings.