10-NANOMETER FILAMENTS AND MITOSIS - MAINTENANCE OF STRUCTURAL CONTINUITY IN DIVIDING ENDOTHELIAL-CELLS
10-NANOMETER FILAMENTS AND MITOSIS - MAINTENANCE OF STRUCTURAL CONTINUITY IN DIVIDING ENDOTHELIAL-CELLS
复制标题
DOI：
10.1073/pnas.76.7.3372
复制
发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
BLOSE, SH
中科院分区：
文献类型：
--
作者：
BLOSE, SH
用间接免疫荧光法研究了10 nm细丝在豚鼠血管内皮细胞有丝分裂的不同阶段的行为。间期细胞包含一圈10纳米的细丝，环绕着细胞核，并保持在与底物平行的平面上。在分裂前期和中期，细胞逐渐变圆，10 nm的细丝环呈波浪形，但仍为闭合结构。随着后期的进展，环被拉长成一个包含纺锤体和染色体的长方形。在末期晚期，胞质分裂在收缩环的位置将10 nm的细丝裂解成新月体。这些新月体在子代细胞中紧密排列成环。如果每毫升5微克细胞松弛素B抑制胞质分裂，则10 nm细丝的切割被阻止，子核仍被母核包围。在有丝分裂过程中，10纳米细丝的排列没有发生重大的解体。与其他主要的细胞质结构，腹侧微丝束和细胞质微管在有丝分裂过程中分解和重组相比，10 nm的微丝在整个过程中保持完整。这些细丝可能在细胞器和结构蛋白的运输中发挥作用，并为子细胞提供关于这些元件在微梁晶格中放置和组装的拓扑信息。
By indirect immunofluorescence the behavior of the 10-nm filaments was studied at various stages of mitosis in guinea pig vascular endothelial cells. Interphase cells contain a ring of 10-nm filaments that encircles the nucleus and is maintained in a plane parallel to the substrate. During prophase and metaphase the cells round up and the 10-nm filament ring becomes wavy though still a closed structure. As anaphase progresses the ring elongates into a rectangle that contains the spindle apparatus and chromosomes. In late telophase, cytokinesis cleaves the 10-nm filaments into crescents at the site of the contractile ring. These crescents close into rings in the daughter cells. If cytokinesis is inhibited with 5 .mu.g of cytochalasin B per ml, then cleavage of the 10-nm filaments is blocked and the daughter nuclei remain surrounded by the parent ring. At no point during mitosis does the array of 10-nm filaments undergo major disassembly. Compared to other major cytoplasmic structures, ventral microfilament bundles and cytoplasmic microtubules, which disassemble and reassemble during mitosis, 10-nm filaments remain intact throughout this process. These filaments may function in transport of organelles and structural proteins and provide the daughter cells with topological information about placement and assembly of these elements within the microtrabecular lattice.