Basic fibroblast growth factor promotes extension of regenerating axons of peripheral nerve. In vivo experiments using a Schwann cell basal lamina tube model
Basic fibroblast growth factor promotes extension of regenerating axons of peripheral nerve. In vivo experiments using a Schwann cell basal lamina tube model
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DOI：
10.1023/a:1015410023132
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发表时间：
1997-08-01
期刊：
影响因子：
--
通讯作者：
Ide, C
中科院分区：
文献类型：
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作者：
Fujimoto, E;Mizoguchi, A;Ide, C
雪旺细胞基膜管作为周围神经轴突再生的吸引管道。在本研究中，通过使用由原位冷冻处理大鼠隐神经制备的基膜管，外源性应用碱性成纤维细胞生长因子（bFGF）对周围神经再生的影响进行了研究，bFGF给药后2和5天。再生的轴突通过光镜和电镜观察，使用PGP9.5-免疫组织化学对轴突进行特异性染色。此外，bFGF及其受体（FGF受体-1）的定位通过免疫组织化学分别使用抗bFGF抗体和抗FGF受体-1抗体进行了检查。再生轴突在bFGF给药段中比在bFGF未给药的对照段中进一步延伸。电子显微镜显示再生的轴突生长出来，没有伴随着雪旺细胞。bFGF处理的神经节段和对照神经节段之间有关血管生成和雪旺细胞迁移的发现非常相似。bFGF免疫反应未检测到在控制神经节段。与此相反，bFGF-免疫反应性检测基板管以及再生轴突的质膜上面对基板在bFGF治疗的神经节段长达5天后，这表明外源性bFGF可以保留在基板数天后，管理。在再生轴突的质膜上检测到FGF受体，它们覆盖基底层。这些结果表明，bFGF可以通过直接影响轴突而不是通过雪旺细胞或血管生成来促进早期再生轴突的延伸。
Schwann cell basal lamina tubes serve as attractive conduits for regeneration of peripheral nerve axons. In the present study, by using basal lamina tubes prepared by in situ freeze-treatment of rat saphenous nerve, the effects of exogenously applied basic fibroblast growth factor (bFGF) on peripheral nerve regeneration was examined 2 and 5 days after bFGF administration. Regenerating axons were observed by light and electron microscopy using PGP9.5-immunohistochemistry for specific staining of axons. In addition, the localizations of bFGF and its receptor (FGF receptor-1) were examined by immunohistochemistry using anti-bFGF antibody and anti-FGF receptor-1 antibody, respectively. Regenerating axons extended further in the bFGF-administered segment than in the bFGF-untreated control segment. Electron microscopy showed that regenerating axons grew out unaccompanied by Schwann cells. Findings concerning angiogenesis and Schwann cell migration were very similar between the bFGF treated and control nerve segment. bFGF-immunoreactivity was not detected in the control nerve segment. In contrast, bFGF-immunoreactivity was detected on the basal lamina tubes as well as on the plasmalemma of regenerating axons facing the basal lamina in the bFGF treated nerve segment up to 5 days after administration, suggesting that exogenous bFGF can be retained in the basal lamina for several days after administration. FGF receptor was detected on the plasma membrane of regenerating axons where they abutted the basal lamina. These results indicate that bFGF could promote the extension of early regenerating axons by directly influencing the axons, but not via Schwann cells or angiogenesis.