Recent advances in bio-orthogonal and dynamic crosslinking of biomimetic hydrogels.
Recent advances in bio-orthogonal and dynamic crosslinking of biomimetic hydrogels.
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DOI：
10.1039/d0tb01429j
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发表时间：
2020-09-21
期刊：
影响因子：
--
通讯作者：
Lin CC
中科院分区：
文献类型：
--
作者：
Arkenberg MR;Nguyen HD;Lin CC
近年来，动态“点击”水凝胶已被应用于许多生物医学应用。由于温和的，细胞相容的，和高度特异性的反应动力学，大量的正交手柄已被开发用于制造动态水凝胶，以促进'4D'细胞培养。正交“点击”化学的交联反应的高度可调性使自下而上的方法能够在人工细胞外基质中安装特定的仿生。除了点击化学，高度特异性的酶反应也越来越多地用于网络交联和水凝胶性质的时空控制。另一方面，共价适应性化学已被用于概括生物组织的粘弹性组分，并用于配制自愈合和剪切稀化水凝胶。这三类化学的共同特征（即，正交点击化学、酶促反应和共价适应性化学）是它们可以在环境和水性条件下进行，这是维持原位细胞包封和凝胶化后网络性质改性的细胞活力的先决条件。由于它们的正交性，不同的化学物质也可以顺序地应用，以提供额外的生物化学和机械控制来引导细胞行为。在此，我们回顾了最近的进展，在使用正交点击化学，酶促反应，共价适应性化学的动态可调和仿生水凝胶的发展。本文综述了近年来通过不可逆点击化学、酶促反应和共价自适应网络交联的生物正交和动态水凝胶的研究进展。
In recent years, dynamic, ‘click’ hydrogels have been applied in numerous biomedical applications. Owing to the mild, cytocompatible, and highly specific reaction kinetics, a multitude of orthogonal handles have been developed for fabricating dynamic hydrogels to facilitate ‘4D’ cell culture. The high degree of tunability in crosslinking reactions of orthogonal ‘click’ chemistry has enabled a bottom-up approach to install specific biomimicry in an artificial extracellular matrix. In addition to click chemistry, highly specific enzymatic reactions are also increasingly used for network crosslinking and for spatiotemporal control of hydrogel properties. On the other hand, covalent adaptable chemistry has been used to recapitulate the viscoelastic component of biological tissues and for formulating self-healing and shear-thinning hydrogels. The common feature of these three classes of chemistry (i.e., orthogonal click chemistry, enzymatic reactions, and covalent adaptable chemistry) is that they can be carried out under ambient and aqueous conditions, a prerequisite for maintaining cell viability for in situ cell encapsulation and post-gelation modification of network properties. Due to their orthogonality, different chemistries can also be applied sequentially to provide additional biochemical and mechanical control to guide cell behavior. Herein, we review recent advances in the use of orthogonal click chemistry, enzymatic reactions, and covalent adaptable chemistry for the development of dynamically tunable and biomimetic hydrogels. This review highlight recent advances in bio-orthogonal and dynamic hydrogels crosslinked by irreversible click chemistry, enzymatic reactions, and covalent-adaptable network.
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