Evolution of S-domain receptor-like kinases in land plants and origination of S-locus receptor kinases in Brassicaceae.
Evolution of S-domain receptor-like kinases in land plants and origination of S-locus receptor kinases in Brassicaceae.
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陆地植物中S结构域受体样激酶的进化和十字花科中S位点受体激酶的起源
DOI：
10.1186/1471-2148-13-69
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发表时间：
2013-03-19
影响因子：
3.4
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Xing S;Li M;Liu P
S结构域的丝氨酸/苏氨酸受体样蛋白(SRLKs)是植物受体样蛋白/PELKs家族中最大、扩展最快的亚家族之一。这个亚家族的创始成员，S基因座受体激酶，在十字花科植物的自交不亲和反应中起着雌性决定因子的作用。陆地植物中也普遍存在与SRK的胞外S结构域(命名为S结构域受体样蛋白，SRLPs)或胞内蛋白激活区(命名为S结构域受体样细胞质蛋白，SRLKs)相似的两类蛋白质，表明SRLKs是由这两种蛋白的结构域融合而来的复合分子。结果根据SRLK和SRLKs的分布模式，建立了SRLK进化过程中基因复制/缺失和融合/裂变事件的最大简约度模型。我们将十字花科植物的SRK等位基因纳入系统发育分析，通过识别合适的外围群来推测SRK的起源。结论推测了两个基因融合事件，主要的基因融合事件发生在陆地植物的共同祖先中，几乎所有现存的SRLK都发生在它们的共同祖先中。通过SRKs的分子进化分析，说明了复制SRLKs的功能多样性。我们的发现支持srks起源于两个古老的单倍型，它们是由十字花科共同祖先中的一对串联重复基因通过随机调控的新功能/亚功能而产生的。
BackgroundThe S-domain serine/threonine receptor-like kinases (SRLKs) comprise one of the largest and most rapidly expanding subfamilies in the plant receptor-like/Pelle kinase (RLKs) family. The founding member of this subfamily, the S-locus receptor kinase (SRK), functions as the female determinant of specificity in the self-incompatibility (SI) responses of crucifers. Two classes of proteins resembling the extracellular S domain (designated S-domain receptor-like proteins, SRLPs) or the intracellular kinase domain (designated S-domain receptor-like cytoplasmic kinases, SRLCKs) of SRK are also ubiquitous in land plants, indicating that the SRLKs are composite molecules that originated by domain fusion of the two component proteins. Here, we explored the origin and diversification of SRLKs by phylogenomic methods.ResultsBased on the distribution patterns of SRLKs and SRLCKs in a reconciled species-domain tree, a maximum parsimony model was then established for simultaneously inferring and dating gene duplication/loss and fusion /fission events in SRLK evolution. Various SRK alleles from crucifer species were then included in our phylogenetic analyses to infer the origination of SRKs by identifying the proper outgroups.ConclusionsTwo gene fusion events were inferred and the major gene fusion event occurred in the common ancestor of land plants generated almost all of extant SRLKs. The functional diversification of duplicated SRLKs was illustrated by molecular evolution analyses of SRKs. Our findings support that SRKs originated as two ancient haplotypes derived from a pair of tandem duplicate genes through random regulatory neo-/sub- functionalization in the common ancestor of the Brassicaceae.
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影响因子：
3.1
作者：
Fukai, E;Fujimoto, R;Nishio, T
通讯作者：
Nishio, T
影响因子：
11.6
作者：
Kusaba, M;Dwyer, K;Nasrallah, ME
通讯作者：
Nasrallah, ME
影响因子：
5.6
作者：
Fong, Jessica H.;Geer, Lewis Y.;Bryant, Stephen H.
通讯作者：
Bryant, Stephen H.
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
10.7
作者：
Charlesworth, D;Bartolomé, C;Mable, BK
通讯作者：
Mable, BK