Isolation procedure and characterization of multipotent adult progenitor cells from rat bone marrow.
Isolation procedure and characterization of multipotent adult progenitor cells from rat bone marrow.
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DOI：
10.1007/978-1-60761-691-7_4
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发表时间：
2010
影响因子：
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通讯作者：
K. Subramanian;M. Geraerts;K. Pauwelyn;Yonsil Park;D. J. Owens;M. Muijtjens;Fernando Ulloa-Montoya;Fernando Ulloa-Montoya;Y. Jiang;C. Verfaillie;W. Hu
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文献类型：
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作者：
K. Subramanian;M. Geraerts;K. Pauwelyn;Yonsil Park;D. J. Owens;M. Muijtjens;Fernando Ulloa-Montoya;Fernando Ulloa-Montoya;Y. Jiang;C. Verfaillie;W. Hu
多能成体祖细胞（Multipotent adult progenitor cells, MAPCs）是从小鼠和大鼠骨髓中提取的成体干细胞，于2002年首次被描述（Jiang et al., Nature 418:41- 49,2002），随后（Breyer et al., Exp Hematol 34:1596-1601, 2006; Jiang et al., Exp Hematol 30:896- 904,2002; Ulloa-Montoya et al., Genome Biol 8:R163, 2007）。啮齿类动物MAPC在单细胞水平上分化为内胚层、中胚层和神经外胚层谱系的一些细胞类型的能力使它们成为研究发育过程的有希望的候选细胞。使用贴壁细胞培养分离MAPC，并在大约8-18周后根据形态学选择。在这里，我们描述了一种逐步可重复的方法，从胎儿和成人骨髓中分离大鼠MAPC。我们详细阐述了分离方案的几个方面，包括细胞密度和培养基成分，以及选择和获得潜在MAPC克隆及其表征的方法。
Multipotent adult progenitor cells (MAPCs) are adult stem cells derived from the bone marrow of mouse and rat and were described for the first time in 2002 (Jiang et al., Nature 418:41-49, 2002), and subsequently (Breyer et al., Exp Hematol 34:1596-1601, 2006; Jiang et al., Exp Hematol 30:896-904, 2002; Ulloa-Montoya et al., Genome Biol 8:R163, 2007). The capacity of rodent MAPC to differentiate at the single-cell level into some of the cell types of endoderm, mesoderm, and neuroectoderm germ layer lineages makes them promising candidates for the study of developmental processes. MAPC are isolated using adherent cell cultures and are selected based on morphology after a period of about 8–18 weeks. Here, we describe a step-by-step reproducible method to isolate rat MAPC from fetal and adult bone marrow. We elaborate on several aspects of the isolation protocol including, cell density and medium components, and methods for selecting and obtaining potential MAPC clones and their characterization.