Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones
Recycling of Chinese herb residues by endophytic and probiotic fungus Aspergillus cristatus CB10002 for the production of medicinal valuable anthraquinones
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DOI：
10.1186/s12934-019-1150-9
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发表时间：
2019-06-04
影响因子：
6.4
通讯作者：
Zhu, Xiangcheng
中科院分区：
文献类型：
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作者：
Kong, Wenping;Huang, Chengshuang;Zhu, Xiangcheng
中药的全球流行促进了中草药的繁荣发展，但每年产生的大量中草药残留物成为环境污染和资源浪费的大问题。微生物在自然界物质循环中发挥着重要的作用，而内生菌是一种未被充分开发的微生物资源，与植物有着独特的共生关系，不仅可以发现次生代谢物，还可以实现草本植物残留物的绿色循环利用。结果以市售化正回生口服液（HOL）中草药渣为原料，分别对几种内生菌进行固态发酵，考察其循环利用能力。其中，从中国茯砖茶中分离得到的一种益生菌曲霉（Aspergillus cristatus CB10002）能够回收HOL残留物生产具有药用价值的蒽醌类物质，其中四种化合物，尤其是具有显著抗肥胖活性的柠檬酸酪蛋白，是首次在茯砖茶中发现。随后的定量分析表明，发酵35天后可获得约2.0mg/g的柠檬酸酪蛋白和7.5mg/g的总蒽醌，具有很强的竞争力和经济效益。进一步的营养比较还表明，回收过程确实改善了HOL残留物的营养成分，从而提出了直接将最终剩余物作为堆肥有机肥处理的可能性。结论从茯砖茶中分离得到内生菌和益生菌A. cristatus CB10002，可有效地利用HOL残基生产9种具有药用价值的蒽醌类化合物，其生物合成可能受HOL残基的诱导调节。这些蒽醌具有竞争力的产量，以及最终剩余物的某些堆肥特性，使得微生物回收HOL残留物具有经济效益。研究结果表明，棘棘菌在中草药废弃物的资源化利用中具有广阔的应用前景，为中草药废弃物的可持续增值微生物资源化利用提供了可行的策略。
BackgroundThe global prevalence of traditional Chinese medicine stimulates the prosperous development of herb medicines, but the annual generation of massive herb residues becomes big issues about environmental pollution and waste of resources. Microbes play important roles in the circulation of substances in nature, and endophytes represent an underexplored microbial resource possessing the unique symbiotic relationship with plants, not only for discovery of secondary metabolites, but also for potential green recycling of herb residues.ResultsThe recycling capacities of several endophytic strains were respectively evaluated via solid state fermentation with herb residues of commercial Huazhenghuisheng oral-liquid (HOL). Among them, Aspergillus cristatus CB10002, a probiotic fungus isolated from Chinese Fu-brick tea, was competent to recycle HOL residues for the production of medicinal valuable anthraquinones, in which four of them, especially citreorosein with significant anti-obesity activity, were first discovered in A. cristatus. Subsequent quantitative analysis showed that about 2.0mg/g citreorosein and 7.5mg/g total anthraquinones could be obtained after 35-day fermentation, which was very competitive and economically beneficial. Further nutritional comparisons also revealed that the recycling process indeed ameliorated the nutrients of HOL residues, and thus proposed a possibility to directly dispose the final leftovers as a compost organic fertilizer.ConclusionsThe endophytic and probiotic fungus A. cristatus CB10002 isolated from Chinese Fu-brick tea was screened out to effectively reutilize HOL residues for the production of nine medicinal valuable anthraquinones, whose biosynthesis may be regulated by the induction of HOL residues. The competitive yields of these anthraquinones, as well as the certain composting properties of final leftovers, have made the microbial recycling of HOL residues economically beneficial. Our work demonstrated a promising applied potential of A. cristatus in reutilization of herb residues, and provided a practical strategy for sustainable and value-added microbial recycling of herb residues.