Hybrid image and blood sampling input function for quantification of small animal dynamic PET data
Hybrid image and blood sampling input function for quantification of small animal dynamic PET data
复制标题
DOI：
10.1016/j.nucmedbio.2007.07.010
复制
发表时间：
2007-11-01
影响因子：
3.1
通讯作者：
Welch, Michael J.
中科院分区：
文献类型：
--
作者：
Shoghi, Kooresh I.;Welch, Michael J.
我们描述并验证了混合图像和血液采样 (HIBS) 方法，以导出用于量化 microPET 小鼠数据的输入函数。 HIBS 算法从图像中导出输入函数的峰值，并对其进行校正以进行恢复，而尾部则从 5 到 6 个最佳放置的血液采样点中导出。使用贝塞尔插值算法将最右边的图像峰值数据点链接到最左边的血液采样点。为了评估 HIBS 的表现，4 只小鼠在注射 0.40-0.50 mCi 的 (18)FDG 后接受了 60 分钟的 microPET 成像。在整个成像过程中总共获得了 21 个血液样本（血液样本血浆时间-活性曲线，bsPTAC），以与提议的 HIBS 方法进行比较。使用滤波反投影在心脏上以 2.75 的变焦重建 MicroPET 图像。通过在左心室的 3-4 个横向平面上绘制直径为 3 像素的圆形 ROI 来组成感兴趣的体积区域 (ROI)。当使用 bsPTAC 和 HIBS 作为输入函数时，通过参数估计偏差的动力学模拟来表征性能。与 HIBS 衍生曲线相比，bsPTAC 曲线的峰值由于时间限制和血液采样延迟而扭曲，这影响了血浆和组织之间的双向交换率。结果凸显了使用 bsPTAC 的局限性。然而，HIBS 方法可产生一致的结果，因此可以替代 bsPTAC。 (c) 2007 Elsevier Inc. 保留所有权利。
We describe and validate a hybrid image and blood sampling (HIBS) method to derive the input function for quantification of microPET mice data. The HIBS algorithm derives the peak of the input function from the image, which is corrected for recovery, while the tail is derived from 5 to 6 optimally placed blood sampling points. A Bezier interpolation algorithm is used to link the rightmost image peak data point to the leftmost blood sampling point. To assess the performance of HIBS, 4 mice underwent 60-min microPET imaging sessions following a 0.40-0.50-mCi bolus administration of (18)FDG. In total, 21 blood samples (blood-sampled plasma time-activity curve, bsPTAC) were obtained throughout the imaging session to compare against the proposed HIBS method. MicroPET images were reconstructed using filtered back projection with a zoom of 2.75 on the heart. Volumetric regions of interest (ROIs) were composed by drawing circular ROIs 3 pixels in diameter on 3-4 transverse planes of the left ventricle. Performance was characterized by kinetic simulations in terms of bias in parameter estimates when bsPTAC and HIBS are used as input functions. The peak of the bsPTAC curve was distorted in comparison to the HIBS-derived curve due to temporal limitations and delay in blood sampling, which affected the rates of bidirectional exchange between plasma and tissue. The results, highlight limitations in using bsPTAC. The HIBS method, however, yields consistent results, and thus, is a substitute for bsPTAC. (c) 2007 Elsevier Inc. All rights reserved.