Dermal fibroblast and epidermal keratinocyte functionality on titania nanotube arrays
Dermal fibroblast and epidermal keratinocyte functionality on titania nanotube arrays
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DOI：
10.1016/j.actbio.2011.03.014
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发表时间：
2011-06-01
影响因子：
9.7
通讯作者：
Popat, Ketul C.
中科院分区：
文献类型：
--
作者：
Smith, Barbara S.;Yoriya, Sorachon;Popat, Ketul C.
穿透皮肤深处的经皮植入物被用于许多临床应用，包括假体和牙科植入物。植入物表面和相应皮肤层之间的良好相互作用对于经皮可植入装置的长期成功至关重要，因此，了解皮肤-生物材料相互作用引起的生理反应是至关重要的。最近的研究表明，在纳米级提供地形线索的材料表面可能为增强生物材料集成提供一种可能的解决方案，从而防止生物材料排斥。在这项研究中，二氧化钛纳米管阵列是用一种简单的阳极氧化技术制备的，作为经皮植入设备的潜在接口。用这些纳米管阵列(直径70~90 nm，长度1~1.5微米)评价人真皮成纤维细胞和表皮角质形成细胞的体外功能。通过荧光显微镜成像、细胞活力测定、间接免疫荧光和扫描电子显微镜观察细胞在贴壁、增殖、定向、活性、细胞骨架组织、分化和形态等方面的功能。研究结果表明，在二氧化钛纳米管阵列上，真皮成纤维细胞增多，表皮角质形成细胞黏附、增殖和分化减少。(C)2011 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Transcutaneous implants that penetrate through the depth of the skin are used in numerous clinical applications, including prosthetics and dental implants. Favorable interactions between the implant surface and the respective skin layers are critical for the long-term success of transcutaneous implantable devices, hence, it is essential to understand the physiologic response elicited by skin-biomaterial interactions. Recent studies have shown that material surfaces that provide topographic cues at the nanoscale level may provide one possible solution to enhanced biomaterial integration, thus preventing biomaterial rejection. In this study titania nanotube arrays were fabricated using a simple anodization technique as potential interfaces for transcutaneous implantable devices. The in vitro functionality of human dermal fibroblasts and epidermal keratinocytes were evaluated on these nanotube arrays (diameter 70-90 nm, length 1-1.5 mu m). Cellular functionality in terms of adhesion, proliferation, orientation, viability, cytoskeletal organization, differentiation and morphology were investigated for up to 4 days in culture using fluorescence microscope imaging, a cell viability assay, indirect immunofluorescence and scanning electron microscopy. The results reported in this study indicate increased dermal fibroblast and decreased epidermal keratinocyte adhesion, proliferation and differentiation on titania nanotube arrays. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.