The Trp64Arg polymorphism of the beta3-adrenergic receptor gene is associated with increased small dense low-density lipoprotein in a rural Japanese population: the Mima study.
The Trp64Arg polymorphism of the beta3-adrenergic receptor gene is associated with increased small dense low-density lipoprotein in a rural Japanese population: the Mima study.
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DOI：
10.1016/j.metabol.2007.07.012
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发表时间：
2007-12
期刊：
影响因子：
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通讯作者：
K. Tsuzaki;K. Kotani;Shinji Fujiwara;Y. Sano;Yukiyo Matsuoka;Masayuki Domichi;T. Hamada;A. Shimatsu;N. Sakane
中科院分区：
文献类型：
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作者：
K. Tsuzaki;K. Kotani;Shinji Fujiwara;Y. Sano;Yukiyo Matsuoka;Masayuki Domichi;T. Hamada;A. Shimatsu;N. Sakane
小而致密的低密度脂蛋白(SdLDL)的存在与发生冠状动脉疾病的风险增加密切相关。β3肾上腺素能受体(β3 AR)基因Trp64Arg多态性是肥胖相关性状的遗传标志。然而，这种多态与sdLDL图谱之间的任何可能的关联尚不清楚。本研究旨在探讨日本农村人群低密度脂蛋白(β)3-AR基因多态性对低密度脂蛋白颗粒大小和低密度脂蛋白的影响。对277名受试者进行体重指数、血压、空腹血清胰岛素水平和胰岛素抵抗指数(空腹血糖×空腹胰岛素/405)的测定。采用聚合酶链式反应-限制性片段长度多态性方法对口腔标本进行β-3-AR基因多态性分析。低密度脂蛋白颗粒大小和sdLDL的测定采用LipoPrint系统(Quantimrix，Redondo比奇，CA)上的脂蛋白电泳法。β3-AR等位基因频率为0.19。Arg携带者(Trp/Arg或Arg/Arg)低密度脂蛋白平均粒径小于非Arg携带者(Trp/Trp)(P<0.05)。Arg携带者sdLDL面积百分比高于非Arg携带者(P<0.05)。多元回归分析显示，sdLDL面积百分比与β3-AR基因多态性相关(P<；0.05)，与年龄、性别、体重指数、吸烟和胰岛素抵抗指数无关。本研究结果提示，β3-AR基因多态性在sdLDL升高的遗传易感性中起作用，而与胰岛素抵抗无关。
The presence of small dense low-density lipoprotein (sdLDL) is closely associated with an increased risk of developing coronary artery disease. The Trp64Arg polymorphism of the β3-adrenergic receptor (β3-AR) gene is a genetic marker for obesity-related traits. However, any possible association between this polymorphism and sdLDL profiles is unclear. The objective of this study is to investigate the effects of the polymorphism of the β3-AR gene on LDL particle size and sdLDL in a rural Japanese population. Among 277 subjects, body mass index, blood pressure, fasting serum insulin levels, and insulin resistance index (fasting glucose × fasting insulin/405) were determined. The polymorphism of the β3-AR gene was assessed by polymerase chain reaction–restriction fragment length polymorphism using buccal samples. Low-density lipoprotein particle size and sdLDL were measured with the electrophoretic separation of lipoproteins on the LipoPrint System (Quantimetrix, Redondo Beach, CA). The frequency of the β3-AR allele was 0.19. In Arg carriers (Trp/Arg or Arg/Arg), the mean value of LDL particle size was smaller than that of non-Arg carriers (Trp/Trp) (P < .05). The area percentage of sdLDL was higher in Arg carriers (P < .05) than in non-Arg carriers. A multiple regression analysis showed that the area percentage of sdLDL was correlated with the polymorphism of the β3-AR gene (P < .05), independently of age, sex, body mass index, smoking, and insulin resistance index. The present findings suggest that the β3-AR gene polymorphism plays a role in the genetic predisposition to increased sdLDL, independently of insulin resistance.