Physical Mapping of 5S rDNA in Two Species of Knifefishes: Gymnotus pantanal and Gymnotus paraguensis (Gymnotiformes)
Physical Mapping of 5S rDNA in Two Species of Knifefishes: Gymnotus pantanal and Gymnotus paraguensis (Gymnotiformes)
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DOI：
10.1159/000328998
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发表时间：
2011-01-01
影响因子：
1.7
通讯作者：
Artoni, R. F.
中科院分区：
文献类型：
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作者：
da Silva, M.;Matoso, D. A.;Artoni, R. F.
用5S rDNA探针进行荧光原位杂交，对两种刀鱼(Gymnotus pantanal和Gymnotus Pantanal)的5S rDNA进行了物理定位。还对两个物种基因组的5S rDNA PCR产物进行了测序和比对，以确定非转录间隔区序列(NTS)。两个物种具有不同的5S rDNA基因簇分布模式。Pantanal的核型中观察到两对5S rDNA，而山竹的核型中含有19对这样的核型。这种5S rDNA基因簇的倍增可能是由于转座元件的参与造成的，因为狭尾鲤鱼的NTS长400bp，与鲤科鱼类Labeo Rohita中描述的可移动的转座子Tc1样转座子有很高的同源性(90%)。版权所有(C)2011 S.Karger AG，巴塞尔
Physical mapping of 5S rDNA in 2 species of knifefishes, Gymnotus pantanal and G. paraguensis (Gymnotiformes), was performed using fluorescence in situ hybridization with a 5S rDNA probe. The 5S rDNA PCR product from the genomes of both species was also sequenced and aligned to determine non-transcribed spacer sequences (NTS). Both species under study had different patterns of 5S rDNA gene cluster distribution. While in the karyotype of G. pantanal two 5S rDNA-bearing pairs were observed, the karyotype of G. paraguensis possessed as many as 19 such pairs. Such multiplication of 5S rDNA gene clusters might be caused by the involvement of transposable elements because the NTS of G. paraguensis was 400 bp long with high identity (90%) with a mobile transposable element called Tc1-like transposon, described from the cyprinid fish Labeo rohita. Copyright (C) 2011 S. Karger AG, Basel