Sublingual immunization with an HIV subunit vaccine induces antibodies and cytotoxic T cells in the mouse female genital tract
Sublingual immunization with an HIV subunit vaccine induces antibodies and cytotoxic T cells in the mouse female genital tract
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DOI：
10.1016/j.vaccine.2010.06.033
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发表时间：
2010-08-02
期刊：
影响因子：
5.5
通讯作者：
Anjuere, Fabienne
中科院分区：
文献类型：
--
作者：
Hervouet, Catherine;Luci, Carmelo;Anjuere, Fabienne
预防人类免疫缺陷病毒（艾滋病毒）异性传播的疫苗应在女性生殖道和生殖器官外产生细胞毒性和抗体反应。我们报道，与皮内免疫相反，用HIV-1 gp41和与霍乱毒素B亚基（CTB）偶联的逆转录酶多肽进行舌下免疫可诱导生殖器粘膜中的gp41特异性IgA抗体和抗体分泌细胞，以及逆转录特异性CD8 T细胞。逆转录酶肽与CTB的偶联有利于其由人类树突状细胞交叉呈现给来自HIV+患者的T细胞系。舌下疫苗接种可能是一种很有前途的预防HIV-1异性传播的疫苗策略。(C) 2010 Elsevier Ltd.版权所有。
A vaccine against heterosexual transmission by human immunodeficiency virus (HIV) should generate cytotoxic and antibody responses in the female genital tract and in extra-genital organs. We report that sublingual immunization with HIV-1 gp41 and a reverse transcriptase polypeptide coupled to the cholera toxin B subunit (CTB) induced gp41-specific IgA antibodies and antibody-secreting cells, as well as reverse transcriptase-specific CD8 T cells in the genital mucosa, contrary to intradermal immunization. Conjugation of the reverse transcriptase peptide to CTB favored its cross-presentation by human dendritic cells to a T cell line from an HIV+ patient. Sublingual vaccination could represent a promising vaccine strategy against heterosexual transmission of HIV-1. (C) 2010 Elsevier Ltd. All rights reserved.