Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
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DOI：
10.1073/pnas.240309797
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发表时间：
2000-12-05
影响因子：
11.1
通讯作者：
Shi, S
中科院分区：
文献类型：
--
作者：
Gronthos, S;Mankani, M;Shi, S
出生后生物体中的牙本质修复通过特化细胞成牙本质细胞的活性发生，成牙本质细胞被认为由与牙髓组织相关的尚未确定的前体群体维持。在这项研究中，我们从成人牙髓中分离出一个克隆性的，快速增殖的细胞群。然后将这些DPSC与人骨髓基质细胞（BMSC）（已知的成骨细胞前体）进行比较。虽然它们在体外具有相似的免疫表型，但功能研究表明，DPSC仅产生零星的，但密集的钙化结节，并且不形成脂肪细胞，而BMSC通常在整个粘附细胞层中钙化，并伴有充满脂质的脂肪细胞。当DPSCs被移植到免疫功能低下的小鼠体内时，它们产生了一种牙本质样结构，里面排列着人成牙本质样细胞，包围着牙髓样间质组织。与此相反，BMSCs形成含有骨细胞和表面衬成骨细胞的板层骨，包围具有活跃的造血和脂肪细胞的纤维血管组织。本研究分离了具有形成牙本质/牙髓样复合物能力的出生后人DPSC。
Dentinal repair in the postnatal organism occurs through the activity of specialized cells, odontoblasts, that are thought to be maintained by an as yet undefined precursor population associated with pulp tissue. In this study, we isolated a clonogenic, rapidly proliferative population of cells from adult human dental pulp. These DPSCs were then compared with human bone marrow stromal cells (BMSCs), known precursors of osteoblasts. Although they share a similar immunophenotype in vitro, functional studies showed that DPSCs produced only sporadic, but densely calcified nodules, and did not form adipocytes, whereas BMSCs routinely calcified throughout the adherent cell layer with dusters of lipid-laden adipocytes. When DPSCs were transplanted into immunocompromised mice, they generated a dentin-like structure lined with human odontoblast-like cells that surrounded a pulp-like interstitial tissue. in contrast, BMSCs formed lamellar bone containing osteocytes and surface-lining osteoblasts, surrounding a fibrous vascular tissue with active hematopoiesis and adipocytes. This study isolates postnatal human DPSCs that have the ability to form a dentin/pulp-like complex.