Effects of stress associated with weaning on the adaptive immune system in pigs
Effects of stress associated with weaning on the adaptive immune system in pigs
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DOI：
10.2527/jas.2010-3470
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发表时间：
2012-02-01
影响因子：
3.3
通讯作者：
Almond, G. W.
中科院分区：
文献类型：
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作者：
Kick, A. R.;Tompkins, M. B.;Almond, G. W.
本研究旨在探讨断奶日龄对猪适应性免疫系统特定成分的影响。23头杂交猪随机分为3组，分别在14（14天，n = 8）、21（21天，n = 7）和28（28天，n = 8）日龄断奶。在猪13、15、20、22、27、29和35日龄时采集外周血样本，分析外周血细胞百分比和中性粒细胞、淋巴细胞、T细胞亚群、成熟B细胞的浓度以及血浆皮质醇浓度。断奶各组血浆皮质醇浓度升高（P < 0.001），体重百分比变化降低（P < 0.017）。淋巴细胞浓度在14D组（P = 0.074）和28D组（P = 0.014）表现出治疗效果。尽管不一致，断奶仔猪的淋巴细胞浓度在断奶后的第一天低于留在母猪或断奶年龄较小的猪。28日龄断奶后，成熟B细胞（CD21(+)）和CD4(+)CD8（+）细胞减少（P < 0.05）。不同处理之间还存在其他差异；然而，这种差异显然与断奶无关。根据本研究中使用的免疫学测量，对于任何断奶年龄的适应性免疫系统都没有明显的益处。早期断奶对猪的适应性免疫能力没有负面影响，这是由免疫细胞群的变化决定的。
This study was designed to investigate the effects of weaning age on specific components of the adaptive immune system in pigs. Twenty-three crossbred pigs were randomly assigned to 1 of 3 treatments: weaning at 14 (14D, n = 8), 21 (21D, n = 7), or 28 (28D, n = 8) d of age. Peripheral blood samples, obtained when pigs were 13, 15, 20, 22, 27, 29, and 35 d of age, were analyzed for peripheral blood cell percentages and concentrations of neutrophils, lymphocytes, T cell subsets, mature B cells, and plasma cortisol concentrations. For each of the 3 groups, weaning increased plasma cortisol concentrations (P < 0.001) and reduced BW percentage change (P < 0.017). Lymphocyte concentrations displayed a treatment effect for the 14D (P = 0.074) and 28D (P = 0.014) groups. Albeit inconsistent, lymphocyte concentrations were less in weaned pigs on the day after weaning than in pigs remaining on the sow or weaned at a younger age. Specifically, mature B cells (CD21(+)) and CD4(+)CD8(+) cells decreased (P < 0.05) after weaning at 28 d of age. Other differences occurred among treatments; however, the differences apparently were not associated with weaning. Based upon the immunological measures used in the present study, there was not an explicit benefit to the adaptive immune system for any weaning age. Early weaning did not negatively affect the adaptive immunological competence of pigs as determined by changes in populations of immune cells.