High Prevalence of Macrolide-Resistant Bordetella pertussis and ptxP1 Genotype, Mainland China, 2014-2016
High Prevalence of Macrolide-Resistant Bordetella pertussis and ptxP1 Genotype, Mainland China, 2014-2016
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DOI：
10.3201/eid2512.181836
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发表时间：
2019-12-01
影响因子：
11.8
通讯作者：
Yao, Kaihu
中科院分区：
文献类型：
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作者：
Li, Lijun;Deng, Jikui;Yao, Kaihu
据中国政府称，报告的百日咳病例显著增加，并仍在增加。为了确定百日咳杆菌菌株的遗传相关性，我们比较了来自中国和西方国家的菌株的多位点可变数目串联重复序列分析（MLVA）结果。在335株中国分离株中，最常见的毒力相关基因型为ptxA 1/ptxC 1/ptxP 1/prn 1/fim 2 -1/fim 3A/tcfA 2，北方分离株的毒力相关基因型多于南方分离株。该基因型的分离株对红霉素高度耐药。我们鉴定了36株ptxP 3菌株，主要携带ptxA 1和prn 2（35/36）; ptxP 3菌株对红霉素敏感，在中国北方较少见。对于所有分离株，磺胺甲恶唑/甲氧苄啶MIC均较低，表明应推荐该药用于红霉素耐药B感染患者。百日咳。150株临床分离株的MLVA鉴定出13种MLVA类型，包括3种主要类型。我们的研究结果表明，在中国流行的分离株不同于西方国家。
According to the government of China, reported cases of pertussis have increased remarkably and are still increasing. To determine the genetic relatedness of Bordetella pertussis strains, we compared multilocus variable-number tandem-repeat analysis (MLVA) results for isolates from China with those from Western countries. Among 335 isolates from China, the most common virulence-associated genotype was ptxA1/ptxC1/ptxP1/prn1/fim2-1/fim3A/tcfA2, which was more frequent among isolates from northern than southern China. Isolates of this genotype were highly resistant to erythromycin. We identified 36 ptxP3 strains mainly harboring ptxA1 and prn2 (35/36); ptxP3 strains were sensitive to erythromycin and were less frequently from northern China. For all isolates, the sulfamethoxazole/trimethoprim MIC was low, indicating that this drug should be recommended for patients infected with erythromycin-resistant B. pertussis. MLVA of 150 clinical isolates identified 13 MLVA types, including 3 predominant types. Our results show that isolates circulating in China differ from those in Western countries.